Interactions between subunits of protein kinase CK2 and their protein substrates influences its sensitivity to specific inhibitors

  • Monika Janeczko
  • Maciej Masłyk
  • Ryszard Szyszka
  • Andrea Baier


Five isoforms of CK2 may exist simultaneously in yeast cells: free catalytic subunits CK2α′, CK2α and three holoenzymatic structures composed of αα′ββ′, α2ββ′ and α′2ββ′. Each isolated and purified form exhibits properties typical for CK2, but they differ in substrate specificity as well as in sensitivity to specific modulators. All five isoforms of protein kinase CK2 from Saccharomyces cerevisiae were examined for their binding capacity with ATP/GTP and two commonly used ATP-competitive inhibitors TBB and TBI. Enzymes were tested with protein substrates differently interacting with CK2 subunits: Elf1, Fip1, Svf1, P2B and synthetic peptide. Obtained results show that Km for ATP varies from 2.4–53 μM for Elf1/CK2α′ and Svf1/CK2α, respectively. Similar differences can be seen in case when GTP was used as phosphate donor. The inhibitory effect depends on composition of CK2/substrate complexes. Highest sensitivity to TBB shows all complexes containing αα′ββ′ isoform with Ki values between 0.2 and 1.1 μM. The prospect that TBB and TBI could be utilized to discriminate between different molecular forms of CK2 in yeast cells was examined. Both inhibitors, TBB as well as TBI, decreases cell growth to extents devoting interactions with different CK2 isoforms present in the cell but the presence of β/β′-dimer has a high importance towards sensitivity. Conceivably, a given inhibitor concentration can inhibit only selected CK2-mediated processes in the cell.


Yeast Protein kinase CK2 isoforms ATP-competitive inhibitors 



Protein kinase CK2


Acidic ribosomal protein P2B


mRNA polyadenylation factor


Survival factor


Transcription elongation factor


Synthetic peptide (RRRADDSDDDDD)






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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Monika Janeczko
    • 1
  • Maciej Masłyk
    • 1
  • Ryszard Szyszka
    • 1
  • Andrea Baier
    • 1
  1. 1.Department of Molecular Biology, Institute of BiotechnologyJohn Paul II Catholic University of LublinLublinPoland

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