Attenuation of oxidative stress, inflammation and early markers of tumor promotion by caffeic acid in Fe-NTA exposed kidneys of Wistar rats

Article

Abstract

Iron nitrilotriacetate (Fe-NTA), a chief environmental pollutant, is known for its extensive toxic manifestations on renal system. In the present study, caffeic acid, one of the most frequently occurring phenolic acids in fruits, grains, and dietary supplements was evaluated for its shielding effect against the Fe-NTA-induced oxidative, inflammatory, and pathological damage in kidney. Fe-NTA was administered (9 mg Fe/kg body weight) intraperitoneally to the Wistar male rats on 20th day while caffeic acid was administered orally (20 and 40 mg/kg body weight) before administration of Fe-NTA. The intraperitoneal administration of Fe-NTA-enhanced lipid peroxidation, xanthine oxidase, and hydrogen peroxide generation with reduction in renal glutathione content, antioxidant enzymes, viz., catalase, glutathione peroxidase, and glutathione reductase. A sharp elevation in the levels of myloperoxidase, blood urea nitrogen (BUN), and serum creatinine has also been observed. Tumor promotion markers viz., ornithine decarboxylase (ODC) and [3H] thymidine incorporation into renal DNA were also significantly increased. Treatment of rats orally with caffeic acid (20 and 40 mg/kg body weight) resulted in a significant decrease in xanthine oxidase (P < 0.001), lipid peroxidation (P < 0.001), γ-glutamyl transpeptidase (P < 0.01), and H2O2 (P < 0.01). There was significant recovery of renal glutathione content (P < 0.001) and antioxidant enzymes (P < 0.001). There was also a reversal in the enhancement of renal ODC activity, DNA synthesis, BUN, and serum creatinine (P < 0.001). All these changes were supported by histological observations. The results indicate that caffeic acid may be beneficial in ameliorating the Fe-NTA-induced oxidative damage and tumor promotion in the kidney of rats.

Keywords

Caffeic acid Inflammation ODC Renal injury 

Abbreviations

Fe-NTA

Ferric nitrilotriacetate

MPO

Myloperoxidase

BUN

Blood urea nitrogen

ROS

Reactive oxygen species

GSH

Reduced glutathione

GST

Glutathione-S-transferase

DTNB-50

Dithio-bis 2-nitrobenzoic acid

CDNB

1-Chloro 2,4-dinitrobenzene

NO

Nitric oxide

GR

Glutathione reductase

GSSG

Oxidized glutathione

NADPH

Reduced nicotinamide adenine dinucleotide phosphate

EDTA

Ethylenediamine tetra acetic acid

LDH

Lactate dehydrogenase

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  1. 1.Molecular Carcinogenesis and Chemoprevention Division, Department of Medical Elementology, Faculty of ScienceJamia Hamdard (Hamdard University)New DelhiIndia
  2. 2.Molecular Carcinogenesis and Chemoprevention Division, Department of Toxicology, Faculty of ScienceJamia Hamdard (Hamdard University)New DelhiIndia

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