Molecular and Cellular Biochemistry

, Volume 354, Issue 1–2, pp 283–289 | Cite as

Coupled expression of dipeptidyl peptidase-IV and fibroblast activation protein-α in transformed astrocytic cells

  • Eva Balaziova
  • Petr Busek
  • Jarmila Stremenova
  • Lucie Sromova
  • Evzen Krepela
  • Libuse Lizcova
  • Aleksi Sedo
Article

Abstract

Dipeptidyl peptidase-IV (DPP-IV) and fibroblast activation protein-α (FAP) are speculated to participate in the regulation of multiple biological processes, because of their unique enzymatic activity, as well as by non-hydrolytic molecular interactions. At present, the role of DPP-IV and FAP in the development and progression of various types of tumors, including glioblastoma, is intensively studied, and their functional crosstalk is hypothesized. In this article, we describe the correlative expression of DPP-IV and FAP mRNA in primary cell cultures derived from human glioblastoma and associated expression dynamics of both molecules in astrocytoma cell lines depending on culture conditions. Although the molecular mechanisms of DPP-IV and FAP co-regulations remain unclear, uncoupled expression of transgenic DPP-IV and the endogenous FAP suggests that it occurs rather at the transcriptional than at the posttranscriptional level. Understanding of the expressional and functional coordinations of DPP-IV and FAP may help clarify the mechanisms of biological roles of both molecules in transformed astrocytic cells.

Keywords

DASH Dipeptidyl peptidase-IV Fibroblast activation protein-α Glioblastoma 

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Eva Balaziova
    • 1
  • Petr Busek
    • 1
  • Jarmila Stremenova
    • 1
  • Lucie Sromova
    • 1
  • Evzen Krepela
    • 1
  • Libuse Lizcova
    • 2
  • Aleksi Sedo
    • 1
  1. 1.Institute of Biochemistry and Experimental Oncology of the 1st Faculty of MedicineCharles University in PraguePrague 2Czech Republic
  2. 2.Institute of Clinical Biochemistry and Laboratory Diagnostics of the 1st Faculty of MedicineCharles University in Prague and General University Hospital in PraguePrague 2Czech Republic

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