Molecular and Cellular Biochemistry

, Volume 354, Issue 1–2, pp 67–75 | Cite as

Induction of pluripotent stem cells transplantation therapy for ischemic stroke

  • Mei Jiang
  • Lei Lv
  • Haifeng Ji
  • Xuelian Yang
  • Wei Zhu
  • Liying Cai
  • Xiaju Gu
  • Changfeng Chai
  • Shu Huang
  • Jian Sun
  • Qiang Dong
Article

Abstract

Stroke can cause permanent neurological damage, complications, and even death. However, there is no treatment exists to restore its lost function. Human embryonic stems transplantation therapy was a novel and potential therapeutic approach for stroke. However, as we have seen, the ethical controversy pertains to embryonic stem cell research. Human induced pluripotent stem cells (iPSCs) are the latest generation of stem cells that may be a solution to the controversy of using embryonic cells. In our study, we generated iPSCs from adult human fibroblasts by introduction of four defined transcription factors (Oct4, Sox2, Nanog, and Lin-28). And then, we investigated the efficacy of iPSCs transplantation therapy for stroke on the animal models of middle cerebral artery occlusion. Surprisingly, we found that transplanted iPSCs migrated to injured brain areas, and differentiated into neuron-like cells successfully. After 4–16 days iPSCs grafting, sensorimotor function of rats has been improved significantly. In one word, we may prove that iPSCs therapy in stroke to be an effective form of treatment.

Keywords

Brain ischemia Induced pluripotent stem cells Fibroblasts Transcription factors Transplantation therapy 

Abbreviations

CNS

Central nervous system

ES

Embryonic stem

%HLV

Percentage hemisphere lesion volume

HESC

Human embryonic stem cells

iPSCs

Induced pluripotent stem cells

MCAO

Middle cerebral artery occlusion

SCNT

Somatic cells nuclear transfer

TTC

2,3,5-Triphenyltetrazolium chloride

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Mei Jiang
    • 1
  • Lei Lv
    • 2
  • Haifeng Ji
    • 1
  • Xuelian Yang
    • 1
  • Wei Zhu
    • 1
  • Liying Cai
    • 1
  • Xiaju Gu
    • 1
  • Changfeng Chai
    • 1
  • Shu Huang
    • 1
  • Jian Sun
    • 1
  • Qiang Dong
    • 2
  1. 1.Department of NeurologyShanghai Pudong New Area Gongli HospitalShanghaiPeople’s Republic of China
  2. 2.Departments of NeurologyHuashan Hospital, Fudan UniversityShanghaiPeople’s Republic of China

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