Molecular and Cellular Biochemistry

, Volume 349, Issue 1–2, pp 205–212

Attenuation of stress-induced gastric lesions by lansoprazole, PD-136450 and ranitidine in rats

  • S. I. Chandranath
  • S. M. A. Bastaki
  • A. D’Souza
  • A. Adem
  • J. Singh
Article
  • 157 Downloads

Abstract

Combining restraint with cold temperature (4°C) consistently induces gastric ulceration in rats after 3.5 h. The cold restraint-stress (CRS) method provides a suitable model for acute ulcer investigations. This study compares the antiulcer activities of lansoprazole (a proton pump inhibitor), PD-136450 (CCK2/gastrin receptor antagonist) and ranitidine (histamine H2 receptor antagonist) on CRS-induced gastric ulcers in rats. The results have shown that lansoprazole, which is a potent anti-secretory agent, provides complete protection in this model of ulcer formation. The use of indomethacin pretreatment to inhibit the prostaglandin (PG) synthesis and NG-nitro l-arginine methyl ester (L-NAME) pretreatment to inhibit nitric oxide synthase did not alter the lansoprazole-induced inhibition of ulcer index obtained in the untreated Wistar rats indicating that these two systems were not involved in the activation of lansoprazole. PD-136450, an effective anti-secretory agent against gastrin- but not dimaprit-induced stimulation, evoked a dose-dependent inhibition of CRS-induced gastric ulcers. The results show that both PG and nitric oxide pathways can influence the inhibitory effect of PD-136450 against CRS-induced gastric ulcer. The antiulcer activities of both lansoprazole and PD-136450 were compared to that of ranitidine. The results showed that ranitidine was more potent than lansoprazole and PD-136450 in inhibiting CRS-induced gastric ulcers and its effect was shown to be influenced by PG as well as nitric oxide synthase. The results of this study have demonstrated that although lansoprazole, PD-136450 and ranitidine were protective against CRS-induced gastric ulcers, the antiulcer activities of PD-136450 and ranitidine involved both PG and nitric oxide pathways, while lansoprazole acted independently of these two systems during CRS.

Keywords

Rats Ulcers Stress Lansoprazole Ranitidine PD-136450 

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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • S. I. Chandranath
    • 1
  • S. M. A. Bastaki
    • 1
  • A. D’Souza
    • 2
    • 3
  • A. Adem
    • 1
  • J. Singh
    • 2
    • 3
  1. 1.Department of Pharmacology, Faculty of Medicine & Health SciencesUAE UniversityAl AinUnited Arab Emirates
  2. 2.School of Forensic and Investigative SciencesUniversity of Central LancashirePrestonEngland, UK
  3. 3.School of Pharmacy and Biomedical SciencesUniversity of Central LancashirePrestonEngland, UK

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