Molecular and Cellular Biochemistry

, Volume 347, Issue 1, pp 145–155

Spatial and temporal expression of hypoxia-inducible factor-1α during myogenesis in vivo and in vitro


DOI: 10.1007/s11010-010-0622-3

Cite this article as:
Wagatsuma, A., Kotake, N. & Yamada, S. Mol Cell Biochem (2011) 347: 145. doi:10.1007/s11010-010-0622-3


We investigated the spatial and temporal expression patterns of hypoxia-inducible factor-1α (HIF-1α) during muscle regeneration and myogenesis in a C2C12 cell culture system. The expression of HIF-1α synchronized with that of myogenic regulatory genes during muscle regeneration at both the mRNA and protein levels. The HIF-1α protein was localized in the nuclei of newly formed regenerating myofibers in three different muscle injury models, including freezing, bupivacaine injection, and muscular dystrophy. In myogenic cell culture, the HIF-1α protein was localized in the nucleus and cytoplasm of the majority of myoblasts and myotubes. HIF-1α protein expression decreased concomitant with the increased expression of MyoD and myogenin proteins after the induction of myogenic differentiation. We investigated the adaptive response of myoblasts to hypoxia-like conditions induced by treatment of cobalt chloride. This treatment allowed HIF-1α to accumulate and translocate to the nucleus to activate transcription of its target genes, suggesting that myoblasts adapted to acute hypoxia-like conditions through enhancing an HIF-1-dependent pathway. Our results provide insight into the possible involvement of HIF-1α in myogenesis in vivo and in vitro.


Hypoxia-inducible factor-1α Muscle regeneration Myogenesis 

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  1. 1.Department of Life Sciences, Graduate School of Arts and SciencesThe University of TokyoTokyoJapan
  2. 2.Department of Advanced Interdisciplinary Studies (AIS), Graduate School of EngineeringThe University of TokyoTokyoJapan

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