Molecular and Cellular Biochemistry

, Volume 345, Issue 1–2, pp 77–89 | Cite as

Vascular endothelial growth factor-C promotes the growth and invasion of gallbladder cancer via an autocrine mechanism

  • Yanling Chen
  • Lei Jiang
  • Feifei She
  • Nanhong Tang
  • Xiaoqian Wang
  • Xiujin Li
  • Shenghua Han
  • Jinhai Zhu


Vascular endothelial growth factor-C (VEGF-C) has a well-defined action on neoplastic lymphangiogenesis and angiogenesis through VEGF receptor-3 (VEGFR-3) and VEGFR-2, respectively, which are generally expressed in endothelial cells. The function of the VEGF-C/receptors pathway in tumor cell types is largely unknown. In this study, we examined the expression and role of VEGF-C/receptors in gallbladder cancer (GBC) cells. We examined the expression of VEGF-C in 50 surgical specimens from gallbladder cancer and three human gallbladder cancer cell lines. Both siRNA and neutralizing antibody to deplete the expression of VEGF-C were used to characterize the biological effect of VEGF-C in GBC NOZ cells. Furthermore, we examined the expression of its receptors, VEGFR-3 and VEGFR-2, in three human GBC cell lines. Our results are as follows: The expression of VEGF-C in the invasive marginal portion was significantly higher than the expression in the central portions. All the three GBC cell lines expressed VEGF-C. Treatment of NOZ cells with VEGF-C siRNA or a neutralizing antibody suppressed cell proliferation and invasion. Moreover, all the three GBC cell lines expressed VEGFR3, but only the NOZ cells expressed VEGFR-2 mRNA. Treatment of NOZ cells with a VEGFR-3 neutralizing antibody suppressed cell invasion, but treatment of NOZ cells with a VEGFR-2 neutralizing antibody suppressed cell proliferation and invasion. In conclusion, GBC cells express both VEGF-C and its receptors. VEGF-C may have a role in the progressive growth and invasion of human GBC through an autocrine mechanism.


Gallbladder cancer VEGF-C Receptors siRNA Autocrine Proliferation Invasion 



This study was supported by the Key Sci-Tech Research Foundation of Fujian Province in China (No.2009Y0021), and the Key Project of Fujian Medical University in China (No. 09ZD017).

Conflict of interests



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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Yanling Chen
    • 1
    • 2
  • Lei Jiang
    • 1
    • 2
  • Feifei She
    • 3
  • Nanhong Tang
    • 1
  • Xiaoqian Wang
    • 1
  • Xiujin Li
    • 1
  • Shenghua Han
    • 2
  • Jinhai Zhu
    • 2
  1. 1.Hepatobiliary Surgery Institute, Union HospitalFujian Medical UniversityFuzhouChina
  2. 2.Department of Hepatobiliary Surgery, Union HospitalFujian Medical UniversityFuzhouChina
  3. 3.Research Center for Molecular Medicine, The Key Laboratory of Infection and Oncology of Universities in Fujian ProvinceFujian Medical UniversityFuzhouChina

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