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Molecular and Cellular Biochemistry

, Volume 344, Issue 1–2, pp 277–284 | Cite as

Changes in mitochondrial dynamics during amyloid β-induced PC12 cell apoptosis

  • Xiaoyu Liu
  • Linqing Feng
  • Ming Yan
  • Kedi Xu
  • Yu Yu
  • Xiaoxiang ZhengEmail author
Article

Abstract

Changes in mitochondrial morphology and dynamics influence mitochondrial function and ultimately damage neurons in Alzheimer’s disease (AD). Amyloid β (Aβ) is a major factor in the pathogenesis of AD. Although it has been proved that Aβ can affect the dynamics of mitochondria, there is little known on the precise dynamic process. Thus, MTT, Hoechst 33342, and Annexin V/PI analysis were used to study Aβ25–35 neurotoxity on PC12 cells, live cell station and image processing were applied to study the moving parameters and characters of mitochondria. We also studied changes of mitochondrial membrane potential and reactive oxygen species production. The results showed that long-term exposure of PC12 cells to Aβ25–35 resulted in increase of mitochondrial number and decrease of mitochondrial length and size, which presented fluctuated during early time and dramatic changes occurred after 6 h. Low concentration exposure caused little mitochondrial changes before 24 h while short time exposure induced mitochondrial fragmentation that could be recovered to normal. Mitochondrial membrane potential dissipation and reactive oxygen species production were observed, as well as apparent cell apoptosis with significant morphological changes. These data suggest that mitochondrial fission can be reversed during Aβ25–35-induced PC12 cell apoptosis, depending on the concentration and exposure time of Aβ25–35, which may be helpful in AD prevention and therapy.

Keywords

Amyloid β Mitochondrial dynamics Live cell station Apoptosis PC12 cells 

Notes

Acknowledgments

This study was supported by the Key Laboratory for Biomedical Engineering of the Ministry of China; the Economic and Trade Commission of Zhejiang Province; and the Key Laboratory of Chinese Medicine Screening, Exploitation and Medicinal Effectiveness Appraise for Cardio-cerebral Vascular and Nervous System of Zhejiang Province. We thank Kurtis Feng for critical reading of the manuscript.

Supplementary material

Supplementary material 1 (MPG 11162 kb)

Supplementary material 2 (MPG 15078 kb)

Supplementary material 3 (MPG 10376 kb)

Supplementary material 4 (MPG 8978 kb)

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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Xiaoyu Liu
    • 1
  • Linqing Feng
    • 1
  • Ming Yan
    • 1
  • Kedi Xu
    • 1
  • Yu Yu
    • 1
  • Xiaoxiang Zheng
    • 1
    Email author
  1. 1.Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical EngineeringZhejiang UniversityHangzhouChina

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