Molecular and Cellular Biochemistry

, Volume 344, Issue 1–2, pp 211–215 | Cite as

Regulation of GSK3 isoforms by phosphatases PP1 and PP2A

  • Félix Hernández
  • Elena Langa
  • Raquel Cuadros
  • Jesús AvilaEmail author
  • Nieves Villanueva


Dephosphorylation of phospho GSK3 isoforms, from COS-7 cells, was determined in vitro and in cultured cells in the absence or the presence of okadaic acid and lithium. Our results indicate a preferential dephosphorylation of phospho GSK3α by PP2A phosphatase, whereas dephosphorylation of phospho GSK3β mainly takes place by PP1 phosphatase.


GSK-3 PP1 PP2A Okadaic acid Lithium 



This study was supported by grants from the Comunidad de Madrid (NEURODEGMODELS-CM), the Spanish Comisión Interministerial de Ciencia y Tecnologia, Fundación Centro Investigación Enfermedades Neurológicas (Fundación CIEN), and the CIBER on Neurodegeneration and by institutional grants from the Fundación Ramón Areces.


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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Félix Hernández
    • 1
  • Elena Langa
    • 1
  • Raquel Cuadros
    • 1
  • Jesús Avila
    • 1
    • 2
    Email author
  • Nieves Villanueva
    • 3
  1. 1.Centro de Biologia Molecular “Severo Ochoa” (CSIC-UAM)MadridSpain
  2. 2.CIBERNEDMadridSpain
  3. 3.Centro Nacional de Microbiología, Instituto de Salud Carlos IIIMadridSpain

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