Molecular and Cellular Biochemistry

, Volume 340, Issue 1–2, pp 73–80 | Cite as

Comparative effects of a novel plant-based calcium supplement with two common calcium salts on proliferation and mineralization in human osteoblast cells

  • Ram Sudheer Adluri
  • Lijun Zhan
  • Manashi Bagchi
  • Nilanjana Maulik
  • Gautam Maulik
Article

Abstract

Calcium is an essential mineral to support bone health and serves as a major therapeutic intervention to prevent and delay the incidence of osteoporosis. Many individuals do not obtain the optimum amount of calcium from diets and depend on bioavailable calcium supplements. The present study was conducted to examine the effect of a novel plant-based calcium supplement, derived from marine algae, and contains high levels of calcium, magnesium, and other bone supporting minerals [commercially known as AlgaeCal (AC)], on proliferation, mineralization, and oxidative stress in cultured human osteoblast cells, and compared with inorganic calcium carbonate and calcium citrate salts. Cultured human fetal osteoblast cells (hFOB 1.19) were treated with AC (0.5 mg/ml, fixed by MTT assay), calcium carbonate, or calcium citrate. These cells were harvested after 4 days of treatment for ALP activity, PCNA expression, and DNA synthesis, and 2 days for Ca2+ deposition in the presence and absence of vitamin D3 (5 nM). The ability of AC to reduce H2O2 (0.3 mM)-induced oxidative stress was assessed after 24 h of treatment. ALP activity was significantly increased with AC treatment when compared to control, calcium carbonate, or calcium citrate (4.0-, 2.0-, and 2.5-fold, respectively). PCNA expression (immunocytochemical analysis), DNA synthesis (4.0-, 3.0-, and 4.0-fold, respectively), and Ca2+ deposition (2.0-, 1.0-, and 4.0-fold, respectively) were significantly increased in AC-treated cells when compared with control, calcium carbonate, or calcium citrate treatment. These markers were further enhanced following additional supplementation of vitamin D3 in the AC-treated group cells. AC treatment significantly reduced the H2O2-induced oxidative stress when compared to calcium carbonate or calcium citrate (1.5- and 1.4-fold, respectively). These findings suggest that AC may serve as a superior calcium supplement as compared to other calcium salts tested in the present study. Hence, AC may be developed as a novel anti-osteoporotic supplement in the near future.

Keywords

Calcium supplement Osteoporosis Mineralization Proliferation Oxidative stress DNA synthesis 

Notes

Acknowledgments

This study was supported by M/s. AlgaeCal, Vancouver, Canada. We thank T. Mahesh for his help in finalizing all the figures.

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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Ram Sudheer Adluri
    • 1
  • Lijun Zhan
    • 1
  • Manashi Bagchi
    • 2
  • Nilanjana Maulik
    • 1
  • Gautam Maulik
    • 3
  1. 1.Molecular Cardiology and Angiogenesis Laboratory, Department of SurgeryUniversity of Connecticut Health CenterFarmingtonUSA
  2. 2.NutriTodayBostonUSA
  3. 3.Department of Cancer Biology, Dana Farber Cancer InstituteHarvard Medical SchoolBostonUSA

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