Molecular and Cellular Biochemistry

, Volume 335, Issue 1–2, pp 291–299 | Cite as

Exercise training decreases hepatic SCD-1 gene expression and protein content in rats

  • Siham YasariEmail author
  • Denis Prud’homme
  • Donghao Wang
  • Marek Jankowski
  • Émile Levy
  • Jolanta Gutkowska
  • Jean-Marc Lavoie


Stearoyl-CoA desaturase-1 (SCD-1) is the rate limiting enzyme in the biosynthesis of saturated-derived monounsaturated fats that are the major constituents of very-low-density-lipoproteins-triacylglycerol (VLDL-TAG) and are involved in regulating cellular metabolism. The purpose of this study was to evaluate the effects of an 8-week exercise training program on the hepatic gene expression of this crucial enzyme. Female rats either trained (TR) or kept sedentary (Sed) for 8 weeks were submitted either to standard (SD) diet for 8 or for 6 weeks followed by high-fat (HF; 42% kcal) diet for 2 weeks. The 2-week-high fat feeding resulted in an increase in liver triacylgycerol (TAG), plasma free-fatty-acids (FFA), abdominal fat mass, sterol-regulatory-element-binding protein-1c (SREBP-1c), and carbohydrate-response-element-binding protein (ChREBP) gene expression in liver along with a decrease in SCD-1 gene expression and plasma and liver SCD-1 desaturation index (C16:1/C16:0). Liver TAG, plasma FFA, SREBP-1c mRNA, and SCD-1 desaturation indexes (C16:1/C16:0; C18:1/C18:0) were not changed in liver or in plasma by the training program. Nevertheless, training resulted in an important decrease in fat mass (P < 0.01), hepatic SCD-1 mRNA levels (P < 0.01), and protein content (P < 0.05) in both SD and HF fed rats. It is concluded that despite an absence of decreased liver TAG, exercise training contributes to the proper regulation of fat metabolism by down-regulating hepatic SCD-1 gene expression and protein content.


Liver lipid content Stearoyl-CoA desaturase-1 High-fat diet SCD-1 desaturation index 



This research was supported by the Natural Sciences and Engineering Research Council of Canada (to JM Lavoie), the Canadian Institutes of Health Research (T 0602145.02 to JM Lavoie and D. Prud’homme, MOP-53217, MOP-62901 to J. Gutkowska and M. Jankowski, and MOP-10584, MOP-49433 to E. Levy). The authors thank Dr. Juris Ozols for the gracious gift of the SCD-1 antibody and Mrs Carole Garofalo and Dr. Alain Montoudis for excellent technical assistance with the lipid measurements.


  1. 1.
    Cohen P, Friedman JM (2004) Leptin and the control of metabolism: role for stearoyl-CoA desaturase-1 (SCD-1). J Nutr 134:2455–2463Google Scholar
  2. 2.
    Cohen P, Miyazaki M, Socci ND, Hagge-Greenberg A, Liedtke W, Soukas AA, Sharma R, Hudgins LC, Ntambi JM, Friedman JM (2002) Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science 297:240–243CrossRefPubMedGoogle Scholar
  3. 3.
    Miyazaki M, Kim YC, Gray-Keller MP, Attie AD, Ntambi JM (2000) The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase-1. J Biol Chem 275:30132–30138CrossRefPubMedGoogle Scholar
  4. 4.
    Ntambi JM, Miyazaki M, Stoehr JP, Lan H, Kendziorski CM, Yandell BS, Song Y, Cohen P, Friedman JM, Attie AD (2002) Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci USA 99:11482–11486CrossRefPubMedGoogle Scholar
  5. 5.
    Dobrzyn A, Ntambi JM (2004) The role of stearoyl-CoA desaturase in body weight regulation. Trends Cardiovasc 14:77–81CrossRefGoogle Scholar
  6. 6.
    Dobrzyn P, Dobrzyn A, Miyazaki M, Cohen P, Asilmaz E, Hardie DG, Friedman JM, Ntambi JM (2004) Stearoyl-CoA desaturase-1 deficiency increases fatty acid oxidation by activating AMP-activated protein kinase in liver. Proc Natl Acad Sci USA 101:6409–6414CrossRefPubMedGoogle Scholar
  7. 7.
    Ntambi JM, Miyazaki M (2003) Recent insights into stearoyl-CoA desaturase-1. Curr Opin Lipidol 14:255–261CrossRefPubMedGoogle Scholar
  8. 8.
    Dobrzyn A, Ntambi JM (2005) Stearoyl-CoA desaturase as a new drug target for obesity treatment. Obes Rev 6:169–174CrossRefPubMedGoogle Scholar
  9. 9.
    Sampath H, Ntambi JM (2006) Stearoyl-coenzyme A desaturase 1, sterol regulatory element binding protein-1c and peroxisome proliferator-activated receptor-α: independent and interactive roles in the regulation of lipid metabolism. Curr Opin Clin Nutr Metab Care 9:84–88CrossRefPubMedGoogle Scholar
  10. 10.
    Musso G, Gambino R, Cassader M (2009) Recent insights into hepatic lipid metabolism in non-alcoholic fatty liver disease (NAFLD). Prog Lipid Res 48:1–26CrossRefPubMedGoogle Scholar
  11. 11.
    Jiang G, Li Z, Ellsworth K, Dallas-Yang Q, Wu M, Ronan J, Esau C, Murphy C, Szalkowski D, Bergeron R, Doebber T, Zhang BB (2005) Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1. J Clin Invest 115:1030–1038PubMedGoogle Scholar
  12. 12.
    Gauthier MS, Couturier K, Latour JG, Lavoie JM (2003) Concurrent exercise prevents high-fat diet-induced macrovesicular hepatic steatosis. J Appl Physiol 94:2127–2134PubMedGoogle Scholar
  13. 13.
    Gauthier MS, Couturier K, Charbonneau A, Lavoie JM (2004) Effects of introducing physical training in the course of a 16-week high-fat diet regimen on hepatic steatosis, adipose tissue fat accumulation, and plasma lipid profile. Int J Obes Relat Metab Disord 28:1064–1071CrossRefPubMedGoogle Scholar
  14. 14.
    Chapados NA, Seelaender M, Levy E, Lavoie J-M (2009) Effects of exercise training on hepatic microsomal triglyceride transfer protein content in rats. Horm Metab Res 41:287–293CrossRefPubMedGoogle Scholar
  15. 15.
    Griffiths MA, Baker DH, Novakofski JE, Ji LL (1993) Effects of exercise training on diet-induced lipogenic enzymes and body composition in rats. J Am Coll Nutr 12:155–161PubMedGoogle Scholar
  16. 16.
    Hoffman-Goetz L, MacDonald M (1983) Effect of treadmill exercise on food intake and body weight in lean and obese rats. Physiol Behav 31:343–346CrossRefPubMedGoogle Scholar
  17. 17.
    Schaible TF, Scheuer J (1985) Cardiac adaptations to chronic exercise. Prog Cardiovasc Dis 27:297–324CrossRefPubMedGoogle Scholar
  18. 18.
    Bedford TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV (1979) Maximum oxygen consumption of rats and its changes with various experimental procedures. J Appl Physiol 47:1278–1283PubMedGoogle Scholar
  19. 19.
    Collin P, Chapados N, Dufresne E, Corriveau P, Imbeault P, Lavoie JM (2006) Time course of changes in vitro lipolysis of intra-abdominal fat depots in relation to high-fat diet-induced hepatic steatosis in rats. Br J Nutr 96:268–275CrossRefPubMedGoogle Scholar
  20. 20.
    Gauthier MS, Favier R, Lavoie JM (2006) Time course of the development of non-alcoholic hepatic steatosis in response to high-fat diet-induced obesity in rats. Br J Nutr 95:273–281CrossRefPubMedGoogle Scholar
  21. 21.
    Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509PubMedGoogle Scholar
  22. 22.
    Mainieri D, Summermatter S, Seydoux J, Montani JP, Rusconi S, Russel AP, Boss O, Buchala A, Dulloo AG (2006) A role for skeletal muscle steoryl-CoA desaturase 1 in control of thermogenesis. FASEB J 20:E1170–E1751CrossRefGoogle Scholar
  23. 23.
    Lepage G, Roy CC (1986) Direct transesterification of all classes of lipids in one-step reaction. J Lipid Res 27:114–120PubMedGoogle Scholar
  24. 24.
    Ntambi JM, Miyazaki M (2004) Regulation of stearoyl-CoA desaturases and role in metabolism. Prog Lipid Res 43:91–104CrossRefPubMedGoogle Scholar
  25. 25.
    Rector RS, Thyfault JP, Matthew JL, Morris RT, Borengasser SJ, Uptergrove GM, Chakravarthy MV, Booth FW, Ibdah JA (2008) Cessation of daily exercise dramatically alters precursors of hepatic steatosis in Otsuka Long Evans Tokushima Fatty (OLETF) rats. J Physiol 586:4241–4249CrossRefPubMedGoogle Scholar
  26. 26.
    Rizki G, Arnaboldi L, Gabrielli B, Yan J, Lee GS, Ng RK, Turner SM, Badger TM, Pitas RE, Maher JJ (2006) Mice fed a lipogenic methionine-choline-deficient diet develop hypermetabolism coincident with hepatic suppression of SCD-1. J Lipid Res 47:2280–2290CrossRefPubMedGoogle Scholar
  27. 27.
    Bjorkegren J, Beigneux A, Bergo MO, Maher JJ, Young SG (2002) Blocking the secretion of hepatic very low density lipoproteins renders the liver more susceptible to toxin-induced injury. J Biol Chem 277:5476–5483CrossRefPubMedGoogle Scholar
  28. 28.
    Kotronen A, Seppänen-Laakso T, Westerbacka J, Kiviluoto T, Arola J, Ruskeepää A-L, Oresic M, Yki-Järvinen H (2009) Hepatic stearoyl-CoA desaturase (SCD-1) activity and diacylglycerol but not ceramide concentrations are increased in the non-alcoholic human fatty liver. Diabetes 58:203–208CrossRefPubMedGoogle Scholar
  29. 29.
    Iizuka K, Bruick RK, Liang G, Horton JD, Uyeda K (2004) Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc Natl Acad Sci USA 101:7281–7286CrossRefPubMedGoogle Scholar
  30. 30.
    Miyazaki M, Dobrzyn A, Man WC, Chu K, Sampath H, Kim HJ, Ntambi JM (2004) Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and-independent mechanisms. J Biol Chem 279:25164–25171CrossRefPubMedGoogle Scholar
  31. 31.
    Miyazaki M, Kim YC, Ntambi JM (2001) A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis. J Lipid Res 42:1018–1024PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Siham Yasari
    • 1
    • 2
    Email author
  • Denis Prud’homme
    • 2
  • Donghao Wang
    • 3
  • Marek Jankowski
    • 3
  • Émile Levy
    • 4
  • Jolanta Gutkowska
    • 3
  • Jean-Marc Lavoie
    • 1
  1. 1.Department of KinesiologyUniversity of MontrealMontrealCanada
  2. 2.Behavioural and Metabolic Research Unit, Montfort Hospital and School of Human Kinetics, Faculty of Health SciencesUniversity of OttawaOttawaCanada
  3. 3.Research Center, Cardiovascular Biochemistry Laboratory, CHUM-Hôtel-DieuUniversity of MontrealMontrealCanada
  4. 4.Research Center, Sainte-Justine Hospital and Department of NutritionUniversity of MontrealMontrealCanada

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