Liv.52 protects HepG2 cells from oxidative damage induced by tert-butyl hydroperoxide
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Oxidative stress induced by toxicants is known to cause various complications in the liver. Herbal drug such as Liv.52 is found to have hepatoprotective effect. However, the biochemical mechanism involved in the Liv.52 mediated protection against toxicity is not well elucidated using suitable in vitro models. Hence, in the present study, the hepatoprotective effect of Liv.52 against oxidative damage induced by tert-butyl hydroperoxide (t-BHP) in HepG2 cells was evaluated in order to relate in vitro antioxidant activity with cytoprotective effects. Cytotoxicity was measured by MTT assay. Antioxidant effect of Liv.52 was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric-reducing antioxidant power (FRAP) assay, and lipid peroxidation and measurement of non-enzymic and antioxidant enzymes in HepG2 cells exposed to t-BHP over a period of 24 h. The results obtained indicate that t-BHP induced cell damage in HepG2 cells as shown by significant increase in lipid peroxidation as well as decreased levels of reduced glutathione (GSH). Liv.52 significantly decreased toxicity induced by t-BHP in HepG2 cells. Liv.52 was also significantly decreased lipid peroxidation and prevented GSH depletion in HepG2 cells induced by t-BHP. Therefore, Liv.52 appeared to be important for cell survival when exposed to t-BHP. The protective effect of Liv.52 against cell death evoked by t-BHP was probably achieved by preventing intracellular GSH depletion and lipid peroxidation. The results showed protective effect of Liv.52 against oxidative damage induced in HepG2 cells. Hence, taken together, these findings derived from the present study suggest the beneficial effect of Liv.52 in regulating oxidative stress induced in liver by toxicants.
KeywordsLiv.52 Liver Oxidative stress HepG2 cells tert-butyl hydroperoxide Hepatoprotection
Authors thank Executive Director for supporting this study.
- 1.Tiwari AK (2004) Antioxidants: new-generation therapeutic base for treatment of polygenic disorders. Curr Sci 86:1092–1102Google Scholar
- 10.Sandhir R, Gill KD (1999) Hepatoprotective effects of Liv. 52 on ethanol induced liver damage in rats. Indian J Expt Biol 37:762–766Google Scholar
- 22.Ohakawa H, Ohishi U, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem 95:145–149Google Scholar
- 37.Kinjo J, Hirakawa T, Tsuchihashi R, Nagao T, Okawa M, Nohara T, Okabe H (2003) Hepatoprotective constituents in plants. 14 Effects of soyasapogenol B, sophoradiol, and their glucuronides on the cytotoxicity of tert-butyl hydroperoxide to HepG2 cells. Biol Pharm Bull 26:1357–1360CrossRefPubMedGoogle Scholar
- 44.Castell JV, Gomez-Lechon MJ, Ponsoda X, Bort R (1997) In vitro investigation of the molecular mechanisms of hepatotoxicity. In: Castell JV, Gomez-Lechon MJ (eds) In vitro methods in pharmaceutical research. Academic Press, London, pp 375–410Google Scholar