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Molecular and Cellular Biochemistry

, Volume 332, Issue 1–2, pp 161–171 | Cite as

Proteomic studies of rat tibialis anterior muscle during postnatal growth and development

  • Hualin Sun
  • Ting Zhu
  • Fei Ding
  • Nan Hu
  • Xiaosong Gu
Article

Abstract

In this study, a proteomic analysis consisting of two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry was accomplished to investigate the complex protein expression patterns in rat tibialis anterior muscle during postnatal 3-month period. We determined the time-dependent expression alterations of 107 protein spots, among which 53 protein spots were identified. These identified proteins included skeletal contractile proteins, metabolic enzymes, chaperone, intermediate filament, and signal transduction proteins. The time-dependent expression of three proteins, such as Mylpf, desmin, and RKIP, was confirmed by Western blot analysis and immunohistochemistry. The functional implication of these expression changes was also discussed. We further analyzed the linkage and interactions among the differentially expressed proteins (MAPK1, RKIP, AHSG, etc.). Collectively, the results might add to the understanding of the molecular mechanisms regulating postnatal growth and development of rat tibialis anterior muscle.

Keywords

Muscle Growth Development 2-DE MALDI-TOF/TOF MS 

Abbreviations

TA

Tibialis anterior muscles

2-DE

Two-dimensional gel electrophoresis

MALDI-TOF/TOF MS

Matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry

ASB-14

Amidosulfobetaine-14

CHAPS

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

DTT

Dithiothreitol

EDTA

Ethylene diamine tetraacetic acid

PMSF

Phenylmethyl sulfonylfluoride

PVDF

Polyvinylidene difluoride

RKIP

Raf kinase inhibitor protein

SDS-PAGE

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

TBP

Tri-butyl-phosphate

TBST

Tris buffered saline-Tween-20

TFA

Trifluoroacetic acid

Notes

Acknowledgments

This study was supported by Hi-Tech Research and Development Program of China (863 Program, Grant No. 2006AA02A128), National Natural Science Foundation of China (Grant No.30670667) and University of Jiangsu Province Key Technological Research Project of China (Grant No. 05KJA31010). We thank professor Jie Liu for the assistance in manuscript preparation.

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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Hualin Sun
    • 1
  • Ting Zhu
    • 1
  • Fei Ding
    • 1
  • Nan Hu
    • 1
  • Xiaosong Gu
    • 1
  1. 1.Jiangsu Key Laboratory of NeuroregenerationNantong UniversityNantongPeople’s Republic of China

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