Molecular and Cellular Biochemistry

, Volume 327, Issue 1–2, pp 257–266

Lentivirus-mediated RNAi knockdown of insulin-like growth factor-1 receptor inhibits growth, reduces invasion, and enhances radiosensitivity in human osteosarcoma cells

  • Yin-He Wang
  • Zhao-Xia Wang
  • Yong Qiu
  • Jin Xiong
  • Yi-Xin Chen
  • Deng-Shun Miao
  • Wei De
Article

DOI: 10.1007/s11010-009-0064-y

Cite this article as:
Wang, YH., Wang, ZX., Qiu, Y. et al. Mol Cell Biochem (2009) 327: 257. doi:10.1007/s11010-009-0064-y

Abstract

The type 1 insulin-like growth factor receptor (IGF-1R) is essential for tumorigenicity, tumor proliferation, and protection from apoptosis. IGF-1R overexpression has been found in many human cancers including osteosarcoma. To explore its possibility as a therapeutic target for the treatment of osteosarcoma, lentivirus-mediated siRNA was employed to downregulate endogenous IGF-1R expression to study the function of IGF-1R in tumorigenesis and radioresistance of osteosarcoma cells. The IGF-1R expression was persistently and markedly reduced by lentivirus-mediated RNAi. Downregulation of IGF-1R expression in osteosarcoma cells significantly suppressed their growth rates in vitro and reduced the potential of tumorigenicity in vivo. Moreover, the specific downregulation arrested cells in G0/G1 phase of cell cycle and also induced apoptosis which correlated with the activation of Caspase-3. Furthermore, we also observed that suppression of IGF-1R could reduce the invasiveness of osteosarcoma cells and enhance their radiosensitivity. Our study suggested that lentivirus-mediated RNAi silencing targeting IGF-1R could induce potent antitumor activity and radiosensitizing activity in human osteosarcomas.

Keywords

Insulin-like growth factor-1 receptor RNA interference Growth Invasion Radiosensitivity Osteosarcoma 

Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Yin-He Wang
    • 1
    • 3
  • Zhao-Xia Wang
    • 4
  • Yong Qiu
    • 3
  • Jin Xiong
    • 3
  • Yi-Xin Chen
    • 3
  • Deng-Shun Miao
    • 2
  • Wei De
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyNanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.Bone and Stem Cell CentreNanjing Medical UniversityNanjingPeople’s Republic of China
  3. 3.Department of Orthopaedic SurgeryThe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingPeople’s Republic of China
  4. 4.Department of OncologyThe Second Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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