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Molecular and Cellular Biochemistry

, Volume 325, Issue 1–2, pp 187–197 | Cite as

Glycine- and proline-rich glycoprotein regulates the balance between cell proliferation and apoptosis for ACF formation in 1,2-dimethylhydrazine-treated A/J mice

  • Sei-Jung Lee
  • Kye-Taek LimEmail author
Article

Abstract

The objective of this study was to investigate the chemopreventive potentials of glycine- and proline-rich glycoprotein (SNL glycoprotein, 150-kDa) isolated from Solanum nigrum Linne on formation of colonic aberrant crypt foci (ACF) induced by 1,2-dimethylhydrazine (DMH, 20 mg/kg) in A/J mice. Administration of SNL glycoprotein inhibited phosphorylation of extracellular signal-regulated kinase (ERK), expression of colonic proliferating cell nuclear antigen (PCNA), and frequency of colonic ACF in DMH-stimulated mice colon carcinogenesis. In addition, SNL glycoprotein increased expression of cyclin-dependent kinase inhibitors (p21WAF/Cip1 and p27Kip1), whereas reduced expression of precursor form of apoptosis-related proteins [pro-caspase-3 and pro-poly(ADP-ribose)polymerase (PARP)] in the mice. Interestingly, the results in this study revealed that SNL glycoprotein has suppressive effects on activity of nuclear factor-kappa B (NF-κB), whereas it has stimulatory effect on the expression of p53, accompanying inhibitory effects on expression of NF-κBp50, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-6, and tumor necrosis factor (TNF)-α in DMH-stimulated ACF formation. Also, SNL glycoprotein has inhibitory effects on the formation of thiobarbituric acid reactive substances (TBARS), on the production of inducible nitric oxide (NO), and on the release of lactate dehydrogenase (LDH) in the mice plasma. Collectively, our findings in this study suggest that SNL glycoprotein has chemopreventive activity via modulation of cell proliferation and apoptosis in DMH-treated A/J mice.

Keywords

Aberrant crypt foci Apoptosis Cell proliferation Colon carcinogenesis SNL glycoprotein 

Notes

Acknowledgment

This study was financially supported by Biotechnology Research Institute, Chonnam National University in 2008.

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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  1. 1.Molecular Biochemistry Laboratory, Biotechnology Research InstituteChonnam National UniversityKwang-juSouth Korea
  2. 2.Center for the Control of Animal Hazards Using Biotechnology (BK 21)Chonnam National UniversityKwang-juSouth Korea

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