Role of immunoexpression of nitric oxide synthases by Hodgkin and Reed-Sternberg cells on apoptosis deregulation and on clinical outcome of classical Hodgkin lymphoma
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Hodgkin and Reed-Sternberg (H-RS) cells of classical Hodgkin lymphoma (cHL) present an impaired expression of immunoglobulin genes, but escape apoptotic death. We investigated whether nitric oxide synthases (NOS) are expressed by H-RS cells, studied their association with EBV status and the expression of apoptotic proteins, and investigated their relationship to the clinical outcome of 171 patients. NOS1 and NOS2 were expressed in a large number of cases, whereas NOS3 expression was not detected. Positive associations were found between NOS1 and p53, bax and NOS2, bcl-2 and NOS2, bax and p53, and between bax and fasL. Inverse correlations were established between EBV and NOS2 and between EBV and bcl-2. A shorter overall survival (OS) was associated with strong expression of NOS2. In conclusion, NOS are expressed by H-RS cells of cHL.
KeywordsClassical Hodgkin lymphoma Nitric oxide synthases Apoptosis-related proteins Epstein–Barr virus Prognosis
The authors are grateful to Mr. José Ivanildo Neves, Mrs. Sueli Nonogaki, Mrs. Osiris Pereira Santos, Mr. Severino da Silva Ferreira, and Mr. César Eugênio Nascimento Braga for their excellent technical assistance. The present study was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, Grant # 03/06989-2). JV and FAS are researchers of the CNPq (Conselho Nacional de Pesquisas Científicas).
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