The antioxidant property of butin was investigated for cytoprotective effect against H2O2-induced cell damage. This compound showed intracellular reactive oxygen species (ROS) scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, inhibition of lipid peroxidation, and DNA damage. This radical scavenging activity of butin protected cell damage exposed to H2O2. Also, butin reduced the apoptotic cells induced by H2O2, as demonstrated by the decreased DNA fragmentation, apoptotic body formation, and caspase 3 activity. In addition, butin restored the activity and protein expression of cellular antioxidant enzymes, superoxide dismutase (SOD), and catalase (CAT) in H2O2-treated cells. Taken together, these findings suggest that butin protected cells against H2O2-induced cell damage via antioxidant property.
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This research was performed under the program of Basic Atomic Energy Research Institute (BAERI) which is part of the Nuclear R&D Programs and in part from the study of the DNA repair regulation with the disease program [M1063901] funded by the Ministry of Science & Technology of Korea (KOSEF).
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