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Molecular and Cellular Biochemistry

, Volume 316, Issue 1–2, pp 49–55 | Cite as

Cell cycle and activation of CK2

  • Miwako Kato HommaEmail author
  • Yoshimi Homma
Article

Abstract

Casein kinase 2 (CK2) is a highly conserved and ubiquitous eukaryotic Ser/Thr protein kinase. Genetic, biochemical, and cell biological studies have indicated the involvement of this enzyme in the control of cell proliferation and in signal transduction. The regulation of CK2 is not well defined, and it has been considered a constitutively non-regulated protein kinase. However, we show that CK2 activation occurred during the progression of cell cycle in response to FBS stimuli of G0 arrested cells. Importantly, we show that as the downstream target for CK2, the phosphorylation of eukaryotic translation-initiation factor eIF5 by CK2 may play a critical role in cell cycle progression. We find that eIF5 is associated with CK2 when the kinase activity is at the highest level in vivo, and is phosphorylated at Ser389 and Ser390 by CK2. Expression of eIF5 mutants that lack those phosphorylation sites reveals that these mutants have a dominant-negative effect on phosphorylation of endogenous eIF5, as well as a significant reduction in the formation of the mature complex, in the growth rate, and the expression of cell cycle-regulated proteins. Also, a pool of CK2 translocates into the nuclear fraction following its activation during the progression of the cell cycle. Consistent with these findings, we report that CK2 may be involved in the regulation of cell cycle progression through the phosphorylation of a key molecule for translation initiation and of nuclear substrates upon activation of CK2 by itself.

Keywords

Adenomatous polyposis coli CK2 Cell cycle Eukaryotic translation initiation factor 5 Nuclear localization Serine/threonine kinase Translation initiation 

Abbreviations

APC

Adenomatous polyposis coli

CK2

Casein kinase 2

DMEM

Dulbecco’s modified Eagle’s medium

eIF5

Eukaryotic translation initiation factor 5

FBS

Fetal bovine serum

Notes

Acknowledgments

We acknowledge Dr. Ahmed K for the critical reading of the manuscript; Drs. D. Li and E.G. Krebs for many helpful discussions. This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan.

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Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  1. 1.Department of Biomolecular ScienceFukushima Medical University School of MedicineFukushimaJapan

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