Effect of the atypical neuroleptic risperidone on morphology and S100B secretion in C6 astroglial lineage cells

  • André Quincozes-Santos
  • Renata Torres Abib
  • Marina Concli Leite
  • Daiane Bobermin
  • Victorio Bambini-Junior
  • Carlos-Alberto Gonçalves
  • Rudimar Riesgo
  • Carmem Gottfried
Article

Abstract

We investigated the effect of the atypical neuroleptic risperidone on morphology and S100B secretion in C6 glioma cells, considering the putative involvement of astroglial cells in neuropsychiatric disorders. In the presence of high experimental doses of risperidone, C6 cells become stellate, with process-bearing cells and partial retraction of the cell body followed by detachment from the adhesion surface with practically no cell death. These results indicate that risperidone is able to interfere with C6 cell adhesion without toxic effects. RhoA activator LPA prevented the effects of risperidone on cell morphology. From 6 h risperidone induced a statistically significant increment of about 80% in S100B secretion. These data contribute to the proposal that glial cells are targets of risperidone, which could be involved in the therapeutic response of risperidone to improve autism symptoms.

Keywords

Risperidone Atypical antipsychotic Astrocyte C6 cells S100B Morphology Autism 

Notes

Acknowledgments

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and FINEP/ Rede IBN 01.06.0842–00. We would like to thank Ms. Alessandra Heizelmann for technical support with cell culture.

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Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • André Quincozes-Santos
    • 1
  • Renata Torres Abib
    • 1
  • Marina Concli Leite
    • 1
  • Daiane Bobermin
    • 1
  • Victorio Bambini-Junior
    • 1
  • Carlos-Alberto Gonçalves
    • 1
  • Rudimar Riesgo
    • 2
  • Carmem Gottfried
    • 1
    • 2
  1. 1.Departamento de Bioquímica, Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Pervasive Developmental Disorders Program (ProTID), Unidade de Neurologia Pediátrica do Hospital de Clínicas de Porto AlegreUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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