Molecular and Cellular Biochemistry

, Volume 310, Issue 1–2, pp 227–234 | Cite as

External Qi of Yan Xin Qigong induces G2/M arrest and apoptosis of androgen-independent prostate cancer cells by inhibiting Akt and NF-κB pathways

  • Xin Yan
  • Hua Shen
  • Hongjian Jiang
  • Chengsheng Zhang
  • Dan Hu
  • Jun Wang
  • Xinqi Wu
Article

Abstract

Long-term clinical observations and ongoing studies have shown antitumor effects of external Qi of Yan Xin Qigong (YXQG-EQ) that originated from traditional Chinese medicine (TCM). In order to understand the molecular mechanisms underlying the antitumor effects of YXQG-EQ, we investigate the effects of YXQG-EQ on growth and apoptosis in androgen-independent prostate cancer PC3 cells. We found that exposure to YXQG-EQ led to G2/M arrest associated with reduced cyclin B1 expression and apoptosis in PC3 cells. YXQG-EQ treatment inhibited constitutive and epidermal growth factor-induced Akt phosphorylation, basal and TNF-α-induced NF-κB activation, and downregulated anti-apoptotic Bcl-2 and Bcl-xL expression. In contrast, exposure to YXQG-EQ increased phosphorylation of Akt and Erk1/2 in human umbilical vein endothelial cells (HUVEC), and had no cytotoxic effect on either HUVEC or peripheral blood mononuclear cells (PBMC). These results indicate that YXQG-EQ has profound effects on growth and apoptosis of prostate cancer cells by targeting survival pathways including the Akt and NF-κB pathways.

Keywords

Akt NF-κB Prostate cancer G2/M arrest Apoptosis External Qi of Yan Xin Qigong 

Notes

Acknowledgments

This work was supported in part by the Yan Xin Foundation.

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Copyright information

© Springer Science+Business Media, LLC. 2007

Authors and Affiliations

  • Xin Yan
    • 1
    • 2
  • Hua Shen
    • 2
  • Hongjian Jiang
    • 3
  • Chengsheng Zhang
    • 4
  • Dan Hu
    • 5
  • Jun Wang
    • 2
  • Xinqi Wu
    • 3
  1. 1.Institute of Chongqing Traditional Chinese MedicineChongqingChina
  2. 2.New Medical Science Research InstituteNew YorkUSA
  3. 3.Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA
  4. 4.McMaster UniversityHamiltonCanada
  5. 5.Dana-Farber Cancer InstituteHarvard Medical SchoolBostonUSA

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