Molecular and Cellular Biochemistry

, Volume 310, Issue 1–2, pp 241–244 | Cite as

Effect of siRNA targeted against MKK4 on myostatin-induced downregulation of differentiation marker gene expression

  • Zhiqing Huang
  • Keying Zhang
  • Xiaoling Chen
  • Jianghong Meng
  • Daiwen Chen
Article

Abstract

The c-Jun N-terminal kinase (JNK) pathway was reported to be involved in myostatin signaling and MKK4 was suggested as the only upstream kinase for myostatin-induced JNK activation, implying that MKK4 is a suitable target of RNA interference (RNAi) for blocking myostatin activity. The aim of this study was to evaluate the effect of small interfering RNA (siRNA) targeted against MKK4 on myostatin-induced downregulation of differentiation marker gene expression. Real-time quantitative PCR revealed that the level of MKK4 expression was efficiently reduced by MKK4-specific siRNA. Western blot assays showed that knockdown of MKK4 attenuated the myostatin-induced downregulation of MyoD and myogenin expression.

Keywords

Myostatin MKK4 siRNA Differentiation marker gene 

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Copyright information

© Springer Science+Business Media, LLC. 2007

Authors and Affiliations

  • Zhiqing Huang
    • 1
  • Keying Zhang
    • 1
  • Xiaoling Chen
    • 1
  • Jianghong Meng
    • 2
  • Daiwen Chen
    • 1
  1. 1.Institute of Animal NutritionSichuan Agricultural UniversityYaanP.R. China
  2. 2.Department of Nutrition and Food ScienceUniversity of MarylandCollege ParkUSA

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