Molecular and Cellular Biochemistry

, Volume 307, Issue 1–2, pp 141–147 | Cite as

NF-κB controls growth of glioblastomas/astrocytomas

  • Denise Smith
  • Takeshi Shimamura
  • Stephanie Barbera
  • Bruce E. Bejcek
Article

Abstract

NF-κB is a family of transcription factors that have been shown to be elevated in a variety of tumor types and in some cases central to their survival and growth. Here we present evidence that U-87 MG and U-118 MG growth is regulated by NF-κB and controlled by PDGF. NF-κB activity was suppressed by a dominant negative mutant of the human PDGF type β receptor and PDGF-B chain neutralizing antibodies. Creation of cell lines that had inducible expression of shRNAs directed against either c-Rel or RelA inhibited growth almost 90% indicating that NF-κB plays a central role in glioblastoma growth.

Keywords

PDGF NF-κB Glioblastoma 

Notes

Acknowledgements

The authors would like to acknowledge Dr. John Geiser for critical reading of the manuscript and Dr. Todd Barkman for sequencing. This work was funded by grants from the National Institutes of Health and the Elsa U. Pardee Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Denise Smith
    • 1
  • Takeshi Shimamura
    • 2
  • Stephanie Barbera
    • 1
  • Bruce E. Bejcek
    • 1
  1. 1.Department of Biological SciencesWestern Michigan UniversityKalamazooUSA
  2. 2.Department of Medical OncologyDana-Farber Cancer InstituteBostonUSA

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