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Molecular and Cellular Biochemistry

, Volume 306, Issue 1–2, pp 87–94 | Cite as

Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells

  • Natalia Mercer
  • Hafiz Ahmed
  • Susana B. Etcheverry
  • Gerardo R. Vasta
  • Ana Maria Cortizo
Article

Abstract

Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.

Keywords

Advanced glycation end products RAGE Galectin-3 Osteoblasts Apoptosis AGE-receptors Regulation 

Notes

Acknowledgments

This work was partially supported by grants from Universidad Nacional de La Plata, Ministerio de Salud y Acción Social de la Nación (Subsecretaría de Investigación y Tecnología, Beca Ramón Carrillo-Arturo Oñativia) and CICPBA to AMC, and grant R01 GM070589-01 from the NIGMS, National Institutes of Health to GRV. NM is a fellow of CONICET, SBE is a member of the Carrera del Investigador, CONICET, and AMC is a member of the Carrera del Investigador, CICPBA.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Natalia Mercer
    • 1
  • Hafiz Ahmed
    • 2
  • Susana B. Etcheverry
    • 1
  • Gerardo R. Vasta
    • 2
  • Ana Maria Cortizo
    • 1
  1. 1.Cátedra de Bioquímica Patológica, Facultad de Ciencias ExactasUniversidad Nacional de La PlataLa PlataArgentina
  2. 2.Center of Marine BiotechnologyUniversity of Maryland Biotechnology InstituteBaltimoreUSA

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