Molecular and Cellular Biochemistry

, Volume 304, Issue 1–2, pp 167–179 | Cite as

Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells

  • Ai-Xia Zhang
  • Wei-Hua Yu
  • Bao-Feng Ma
  • Xin-Bing Yu
  • Frank Fuxiang Mao
  • Wei Liu
  • Jia-Qing Zhang
  • Xiu-Ming Zhang
  • Shu-Nong Li
  • Ming-Tao Li
  • Bruce T. Lahn
  • Andy Peng Xiang


Human mesenchymal stem cells (hMSC) are a population of multipotent cells that can differentiate into osteoblasts, chondrocytes, adipocytes, and other cells. The exact mechanism governing the differentiation of hMSC into osteoblasts remains largely unknown. Here, we analyzed protein expression profiles of undifferentiated as well as osteogenic induced hMSC using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and peptide mass fingerprinting (PMF) to investigate the early gene expression in osteoblast differentiation. We have generated proteome maps of undifferentiated hMSC and osteogenic induced hMSC on day 3 and day 7. 2-DE revealed 102 spots with at least 2.0-fold changes in expression and 52 differently expressed proteins were successfully identified by MALDI-TOF-MS. These proteins were classified into 7 functional categories: metabolism, signal transduction, transcription, calcium-binding protein, protein degradation, protein folding and others. The expression of some identified proteins was confirmed by further RT-PCR analyses. This study clarifies the global proteome during osteoblast differentiation. Our results will play an important role in better elucidating the underlying molecular mechanism in hMSC differentiation into osteoblasts.


Differential protein expression Human mesenchymal stem cells Osteoblast differentiation Proteomics 2-DE 



Human mesenchymal stem cells


Molecular weight

Supplementary material

11010_2007_9497_MOESM1_ESM.pdf (83 kb)
Fig. S1 The expressions of 7 protein spots on the 2-DE afterosteoblast differentiation. 0 d: undifferentiated hMSC. 3 d, 7 d: days after treating inducing media (PDF 84 kb)


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Ai-Xia Zhang
    • 1
  • Wei-Hua Yu
    • 1
    • 2
  • Bao-Feng Ma
    • 1
  • Xin-Bing Yu
    • 1
  • Frank Fuxiang Mao
    • 1
  • Wei Liu
    • 3
  • Jia-Qing Zhang
    • 1
  • Xiu-Ming Zhang
    • 1
  • Shu-Nong Li
    • 1
  • Ming-Tao Li
    • 3
  • Bruce T. Lahn
    • 1
  • Andy Peng Xiang
    • 1
  1. 1.Center for Stem Cell Biology and Tissue EngineeringSun Yat-sen UniversityGuangzhouChina
  2. 2.Department of Pathophysiology, the School of Preclinical MedicineSun Yat-sen UniversityGuangzhouChina
  3. 3.The Proteomics Laboratory, the School of Preclinical MedicineSun Yat-sen UniversityGuangzhouChina

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