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Molecular and Cellular Biochemistry

, Volume 303, Issue 1–2, pp 19–25 | Cite as

Reactive oxygen species plasmatic levels in ischemic stroke

  • Laura Nanetti
  • Ruja Taffi
  • Arianna Vignini
  • Cinzia Moroni
  • Francesca Raffaelli
  • Tiziana Bacchetti
  • Mauro Silvestrini
  • Leandro Provinciali
  • Laura Mazzanti
Article

Abstract

Oxidative stress is probably one of the mechanisms involved in neuronal damage induced by ischemia-reperfusion, and the antioxidant activity of plasma may be an important factor providing protection from neurological damage caused by stroke-associated oxidative stress. The aim of this study was to investigate the status of oxidative stress, NO and ONOO levels in patients with atherothrombotic and lacunar acute ischemic stroke and iNOS, eNOS and nitrotyrosine expression in the same patients. Plasma ONOO levels were significantly higher in patients than in controls while NO decreases in patients in respect to controls. Densitometric analysis of bands indicated that iNOS and N-Tyr protein levels were significantly higher in patients in respect to controls. This study has highlighted a significant NO decrease in our patients compared with controls and this is most probably due to the increased expression of inducible NO synthase by the effect of thrombotic attack. In fact, the constitutive NO isoforms, which produce small amounts of NO, are beneficial, while activation of the inducible isoform of NO, which produces much more NO, causes injury, being its toxicity greatly enhanced by generation of peroxynitrite. The significant ONOO increase observed in our patients, compared to controls, is most probably due to reaction of NO with O 2 ·− . These findings suggest that free radical production and oxidative stress in ischemic stroke might have a major role in the pathogenesis of ischemic brain injury. Peroxynitrite might be the main marker of brain damage and neurological impairment in acute ischemic stroke.

Keywords

Nitric oxide Peroxynitrite Nitric oxide synthase Plasma Stroke 

Notes

Acknowledgments

The present work was supported by a grant Fondazione Cavaliere del Lavoro Mario Magnetto to L M.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Laura Nanetti
    • 1
  • Ruja Taffi
    • 2
  • Arianna Vignini
    • 1
  • Cinzia Moroni
    • 1
  • Francesca Raffaelli
    • 1
  • Tiziana Bacchetti
    • 1
  • Mauro Silvestrini
    • 2
  • Leandro Provinciali
    • 2
  • Laura Mazzanti
    • 1
  1. 1.Institute of BiochemistryUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Department of Neurological ScienceUniversità Politecnica delle MarcheAnconaItaly

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