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Molecular and Cellular Biochemistry

, Volume 302, Issue 1–2, pp 27–34 | Cite as

Regulation of singlet oxygen-induced apoptosis by cytosolic NADP+-dependent isocitrate dehydrogenase

  • Sun Yee Kim
  • Su Min Lee
  • Jean Kyoung Tak
  • Kyeong Sook Choi
  • Taeg Kyu Kwon
  • Jeen-Woo ParkEmail author
Article

Abstract

Singlet oxygen is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules and it also promotes deleterious processes such as cell death. Recently, we demonstrated that the control of redox balance and the cellular defense against oxidative damage are the primary functions of cytosolic NADP+-dependent isocitrate dehydrogenase (IDPc) through supplying NADPH for antioxidant systems. In this report, we demonstrate that modulation of IDPc activity in HL-60 cells regulates singlet oxygen-induced apoptosis. When we examined the protective role of IDPc against singlet oxygen-induced apoptosis with HL-60 cells transfected with the cDNA for mouse IDPc in sense and antisense orientations, a clear inverse relationship was observed between the amount of IDPc expressed in target cells and their susceptibility to apoptosis. The results suggest that IDPc plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen.

Keywords

Singlet oxygen Isocitrate dehydrogenase HL-60 Apoptosis Antioxidant enzyme 

Notes

Acknowledgment

This work was supported by a grant from the Korea Research Foundation (KRF-2005-070-C00100).

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Sun Yee Kim
    • 1
  • Su Min Lee
    • 1
  • Jean Kyoung Tak
    • 1
  • Kyeong Sook Choi
    • 2
  • Taeg Kyu Kwon
    • 3
  • Jeen-Woo Park
    • 1
    Email author
  1. 1.School of Life Sciences and Biotechnology, College of Natural SciencesKyungpook National UniversityTaeguKorea
  2. 2.Ajou University Medical SchoolSuwonKorea
  3. 3.Keimyung University Medical SchoolTaeguKorea

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