Molecular and Cellular Biochemistry

, Volume 301, Issue 1, pp 83–92

Osteogenic differentiation of adipose derived stem cells promoted by overexpression of osterix

  • Ling Wu
  • Yao Wu
  • Yunfeng Lin
  • Wei Jing
  • Xin Nie
  • Ju Qiao
  • Lei Liu
  • Wei Tang
  • Weidong Tian
Article

DOI: 10.1007/s11010-006-9399-9

Cite this article as:
Wu, L., Wu, Y., Lin, Y. et al. Mol Cell Biochem (2007) 301: 83. doi:10.1007/s11010-006-9399-9

Abstract

Adipose-derived stem cells (ASCs) are considered to be multipotent mesenchymal stem cells that are easily induced to differentiate into functional osteoblasts both in vitro and in vivo. Osterix (Osx) is a zinc finger-containing transcription factor of Sp gene family, which plays important roles in bone development and mineralization. In this study, we hypothesized that overexpression of Osx in murine ASCs would promote their osteogenic differentiation in vitro. A plasmid expressing Osx (pcDNA3.1-Osx) was constructed and applied to transfect monolayers of murine ASCs. Then expression of bone-related genes, nodule formation, proliferation rate, and alkaline phosphatase activity were examined to evaluate the osteogenic potential of ASCs with pcDNA3.1-Osx transfection. Results of RT-PCR and immunohistochemistry showed that pcDNA3.1-Osx transfection enhanced the expression of bone matrix proteins, such as bone sialoprotein, osteocalcin, osteopontin, and Collagen type I in ASCs. At the same time, overexpression of Osx in ASCs enhanced alkaline phosphatase activity and capability to form mineralized nodules, while not inhibited their proliferation rate. These results indicated that pcDNA3.1-Osx transfection promoted the osteogenic differentiation of ASCs, while not affecting their proliferative ability. Since they can be easily isolated and genetically modified, ASCs are hopeful cell sources in the further application of hard tissue engineering.

Keywords

Adipose derived stem cells Osterix Osteogenic differentiation Osteoblasts Overexpression 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Ling Wu
    • 1
  • Yao Wu
    • 1
  • Yunfeng Lin
    • 1
  • Wei Jing
    • 1
  • Xin Nie
    • 1
  • Ju Qiao
    • 2
  • Lei Liu
    • 1
  • Wei Tang
    • 1
  • Weidong Tian
    • 1
  1. 1.Department of Oral and Maxillofacial Surgery, West China College of StomatologySichuan UniversityChengduP.R. China
  2. 2.Department of Othodontics, West China College of StomatologySichuan UniversityChengduP.R. China

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