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Molecular and Cellular Biochemistry

, Volume 295, Issue 1–2, pp 45–52 | Cite as

Role of antioxidant systems, lipid peroxidation, and nitric oxide in postmenopausal osteoporosis

  • Salih OzgocmenEmail author
  • Huseyin Kaya
  • Ersin Fadillioglu
  • Rabia Aydogan
  • Zumrut Yilmaz
Article

Abstract

In this study we assessed activities of antioxidant enzymes, lipid peroxidation end-products, and nitric oxide (NO) levels in women with postmenopausal osteoporosis (PMO). Relationship between oxidative stress parameters and NO levels with bone mineral density (BMD) and clinical variables influencing bone mass and health related quality of life measures was also investigated in women with PMO.

Postmenopausal women (n = 87), aged 40–65, without previous diagnosis or treatment for osteoporosis and independent in daily living activities were included. BMD was measured at the lumbar spine and proximal femur using dual-X-ray absorptiometry (DXA). Erythrocyte catalase (CATe) enzyme activity, erythrocyte and plasma enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and lipid peroxidation end-product malondialdehyde (MDA) and nitrite/nitrate levels, by product of NO were studied. A total of 23 healthy non-porotic women were included as controls.

Women with PMO had significantly lower erythrocyte CATe enzyme activity and higher erythrocyte malondialdehyde (MDAe) and erythrocyte nitric oxide (NOe) levels in comparison to controls whereas erythrocyte SODe and GSH-Px enzyme activity was similar. In plasma, osteporotic women had significantly higher SOD enzyme activity and higher MDA levels whereas similar GSH-Px enzyme activity and NO levels compared to non-porotic controls. Significant correlation was found between erythrocyte SODe, CATe enzyme activity and NOe levels with proximal femur BMD. Some of the quality of life scores as pain, mental, and social functions correlated with antioxidant enzyme activities and NO levels.

Consequently, oxidative stress markers may be an important indicator for bone loss in postmenopausal women. Further researches assessing the oxidative stress markers and NO in bone tissue and changes with anti-osteoporotic drugs would be valuable to better understand the role of free radicals, antioxidants, and NO in the regulation of bone mass.

Keywords

antioxidant enzyme malondialdehyde nitric oxide postmenopausal osteoporosis 

Abbreviations

ROS

Reactive oxygen species

NOS

Nitric oxide synthase

nNOS

Neuronal nitric oxide synthase

iNOS

Inducible nitric oxide synthase

eNOS

Endothelial nitric oxide synthase

PMO

Postmenopausal osteoporosis

DXA

Dual-X-ray absorptiometry

QUALEFFO

Quality of Life Questionnaire of the European Foundation for Osteoporosis-41 item

CTx

Type I cross-linked C-telopeptide

BSO

L-buthionine-(S,R)-sulfoximine

ONOO

Peroxynitrite

CATe

Erythrocyte catalase

SODe

Erythrocyte superoxide dismutase

MDAe

Erythrocyte malondialdehyde

GSH-Pxe

Erythrocyte glutathione peroxidase

NOe

Erythrocyte nitric oxide

BMD

Bone mineral density

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Copyright information

© Springer 2006

Authors and Affiliations

  • Salih Ozgocmen
    • 1
    Email author
  • Huseyin Kaya
    • 1
  • Ersin Fadillioglu
    • 2
  • Rabia Aydogan
    • 1
  • Zumrut Yilmaz
    • 3
  1. 1.Division of Rheumatology, Department of Physical Medicine and Rehabilitation, Faculty of MedicineFirat UniversityElazigTurkey
  2. 2.Department of Physiology, Faculty of MedicineHacettepe UniversityAnkaraTurkey
  3. 3.Department of Physiology, Faculty of MedicineInonu UniversityMalatyaTurkey

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