Molecular and Cellular Biochemistry

, Volume 289, Issue 1–2, pp 185–191

(–)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes

  • Po Yee Chiu
  • Hoi Yan Leung
  • Michel K. T. Poon
  • Duncan H. F. Mak
  • Kam Ming Ko
Article

Abstract

Effects of schisandrin B enantiomers ((+)Sch B and (–)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (–)Sch B at 6.25 μM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (–)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (–)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (–)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.

Keywords

schisandrin B enantiomers glutathione heat shock protein H9c2 cardiomyocytes 

Abbreviation

 

FBS

fetal bovine serum

 

GSH

reduced glutathione

 

Hsp

heat shock protein

 

LDH

lactate dehydrogenase

 

PBS

phosphate-buffered saline

 

Sch B

schisandrin B

 

XO

Xanthine oxidase

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Po Yee Chiu
    • 1
  • Hoi Yan Leung
    • 1
  • Michel K. T. Poon
    • 1
  • Duncan H. F. Mak
    • 1
    • 2
  • Kam Ming Ko
    • 1
  1. 1.Department of BiochemistryThe Hong Kong University of Science & TechnologyHong KongChina
  2. 2.Section of Molecular Hematology and Therapy, Department of Blood and Marrow TransplantationUT MD Anderson Cancer CenterHoustonUSA

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