Molecular and Cellular Biochemistry

, Volume 289, Issue 1–2, pp 1–9 | Cite as

Cardioprotective effect of lycopene in the experimental model of myocardial ischemia-reperfusion injury

  • Pankaj Bansal
  • Suresh Kumar Gupta
  • Shreesh Kumar Ojha
  • Mukesh Nandave
  • Rajan Mittal
  • Santosh Kumari
  • Dharamvir Singh AryaEmail author


The efficacy of lycopene to limit myocardial injury after ischemia and reperfusion was explored in the present study. Adult male albino Wistar rats were divided into three experimental groups and orally received olive oil as vehicle (sham and control I-R) or lycopene 1 mg/kg dissolved in olive oil (lycopene treated group) respectively for 31 days. On the 31st day, animals of the control I-R and lycopene treated groups were subjected to 45 min of occlusion of the LAD coronary artery and were thereafter reperfused for 1 h. The ischemia-reperfusion injury resulted in significant cardiac necrosis, depression in hemodynamics, decline in antioxidant status and rise in lipid peroxidation product levels in the control I-R group as compared to sham control. In histopathological examinations myocardial damage produced after I-R was significantly prevented in the lycopene treated group. Lycopene treatment resulted in preservation of the myocardial antioxidant status and altered hemodynamic parameters as compared to control I-R group. Furthermore, I-R-induced lipid peroxidation was significantly inhibited in the lycopene treated group. These beneficial cardioprotective effects also translated into the functional recovery of the heart. The beneficial effect of lycopene likely results from the suppression of oxidative stress, which results in the reduction of myocardial injury.


lycopene myocardial infarction ischemia reperfusion injury nutritional antioxidants 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Begum R, Singh RB: Prevalence of coronary artery disease and its risk factor in urban population of South and North India. Acta Cardiologica 3: 227–240, 1995Google Scholar
  2. 2.
    Visioli F, Borsani L, Claudio G: Diet and prevention of coronary heart disease: the potential role of phytochemicals. Cardiovasc Res 47: 419–425, 2000CrossRefPubMedGoogle Scholar
  3. 3.
    Hertog MGL, Feskens EJM, Hollam PCH, Katan MB, Kromhout D: Dietary antioxidant flavonoids and risk of coronary heart diseases. The Zutphen elderly Study. Lancet 342: 1007–1020, 1993Google Scholar
  4. 4.
    Miller NJ, Sampson J, Candeias LP, Bramley PM, Rice-Evans CA: Antioxidant activities of carotenes and xanthophylls. FEBS Lett 384: 240–246, 1996CrossRefPubMedGoogle Scholar
  5. 5.
    Bohm F, Tinkler JH, Truscott TG: Carotenoids protect against cell membrane damage by nitrogen dioxide radical. Nat Med 1: 98–99, 1995CrossRefPubMedGoogle Scholar
  6. 6.
    Greening ER, Baron JA, Toteson TD: Clinical trial of antioxidant vitamins to prevent colorectal adenocarcinoma. N Engl J Med 331: 141–147, 1994CrossRefPubMedGoogle Scholar
  7. 7.
    Clinton SK: Lycopene: chemistry, biology, and implications for human health and disease. Nutr Rev 1: 35–51, 1998Google Scholar
  8. 8.
    DiMascio P, Kaiser S, Sies H: Lycopene as the most effective biological carotenoid singlet oxygen scavenger. Arch Biochem Biophys 274: 532–538, 1989CrossRefPubMedGoogle Scholar
  9. 9.
    Krinsky NI: Overview of lycopene, carotenoids, and disease prevention. Proc Soc Exp Biol Med 218: 95–100, 1998PubMedGoogle Scholar
  10. 10.
    Hearse DJ: Prospects for antioxidant therapy in cardiovascular medicine. Am J Med 91: 118S–121S, 1991CrossRefPubMedGoogle Scholar
  11. 11.
    Bernier M, Manning AS, Hearse DJ: Reperfusion arrhythmias: dose-related protection by anti-free radical interventions. Am J Physiol 256: H1344–H1352, 1989PubMedGoogle Scholar
  12. 12.
    Tappia PS, Heta T, Hozaima L, Sandhu MS, Panagia V, Dhalla NS: Role of oxidative stress in catecholamine-induced changes in cardiac sarcolemmal Ca2+ transport. Arch Biochem Biophys 377: 85–92, 2001CrossRefGoogle Scholar
  13. 13.
    Ferrari R, Alfieri O, Curello S, Ceconi S, Cargnoni A, Marzollo P, Pardini A, Caradonna E, Visioli O: Occurrence of oxidative stress during reperfusion of the human heart. Circulation 81: 201–211, 1990PubMedGoogle Scholar
  14. 14.
    Bernier M, Hearse DJ, Manning AS: Reperfusion-induced arrhythmias and oxygen derived free radicals. Circ Res 58: 331–340, 1986PubMedGoogle Scholar
  15. 15.
    Curello S, Ceconi C, Medici D, Ferrari R: Oxidative stress during myocardial ischemia and reperfusion: experimental and clinical evidences. J Appl Cardiol 1: 311–327, 1986Google Scholar
  16. 16.
    Maron MS, Depierre JW, Mannervik B: Level of glutathione, glutathione reductase and glutathione-s-transferase activity in rat lung and liver. Biochem Biophys Acta 82: 67–78, 1979Google Scholar
  17. 17.
    Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte peroxidase. J Lab Clin Med 2: 158, 1967Google Scholar
  18. 18.
    Misra HP, Fridovich I: The oxidation of phenylhydrazine: superoxide and mechanisms. Biochemistry 15: 681–687, 1976CrossRefPubMedGoogle Scholar
  19. 19.
    Aebi H: Catalase. In: H. E. Bergemayer (ed). Methods of Enzymatic Analysis. 2nd ed. Vol. 2, Verlag Chemie, Weinheim, Germany, 1974, pp. 213–215Google Scholar
  20. 20.
    Ohkawa H, Ohishi N, Yagi K: Assay of lipid peroxide in animal tissue by thiobarbituric acid reaction. Anal Biochem 95: 351–358, 1979CrossRefPubMedGoogle Scholar
  21. 21.
    Lamprecht W, Stan F, Weisser H, Heinz F: Determination of creatine phosphate and adenosine triphosphate with creatine kinase. In: H. U. Bergmeyer (ed). Methods of Enzymatic Analysis, Academic Press, New York, 1974, pp. 1776–1778Google Scholar
  22. 22.
    Lowry OH, Rosebrough NJ, Farr AI, Randall RJ: Protein measurements with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951PubMedGoogle Scholar
  23. 23.
    Sevenian A, Hochstein P: Mechanisms and consequences of lipid peroxidation in biological systems. Annu Rev Nutr 5: 365–375, 1985CrossRefPubMedGoogle Scholar
  24. 24.
    Jennings RB, Murry CE, Steenbergen CJR, Reimer KA: Acute myocardial ischemia: development of cell injury in sustained ischemia. Circulation 82: 3–12, 1990Google Scholar
  25. 25.
    Dormandy TL: Free radical oxidation and antioxidants. Lancet 1: 647–650, 1978CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Pankaj Bansal
    • 1
  • Suresh Kumar Gupta
    • 2
  • Shreesh Kumar Ojha
    • 1
  • Mukesh Nandave
    • 1
  • Rajan Mittal
    • 1
  • Santosh Kumari
    • 3
  • Dharamvir Singh Arya
    • 1
    Email author
  1. 1.Department of PharmacologyAll India Institute of Medical SciencesNew DelhiIndia
  2. 2.Delhi Institute of Pharmaceutical Sciences and ResearchNew DelhiIndia
  3. 3.Division of Plant PhysiologyIndian Agriculture Research InstituteNew DelhiIndia

Personalised recommendations