Molecular and Cellular Biochemistry

, Volume 293, Issue 1–2, pp 87–92 | Cite as

Schisandrin B induced antioxidant response is partly mediated by cytochrome P-4502E1 catalyzed reaction in mouse liver

  • Po Yee Chiu
  • Hoi Yan Leung
  • Michel K. T. Poon
  • Susanna S. T. Lee
  • Kam Ming Ko


In order to explore the role of cytochrome P-450 (CYP) 2E1 in schisandrin B (Sch B)-induced antioxidant and heat shock responses, the effects of Sch B treatment on hepatic mitochondrial glutathione antioxidant status (mtGAS) and heat shock protein (Hsp)25/70 expression were compared between wild-type and cyp2e1 knock-out C57B/6N mice. Cyp2e1 knock-out mice exhibited a significantly smaller degree of Sch B-induced enhancement in hepatic mtGAS when compared with the wild-type counterpart. But Hsp25/70 expression induced by Sch B was not affected. Sch B-induced enhancement of mtGAS was corroborated by the increase in hepatic mitochondrial antioxidant capacity, as assessed by in vitro measurement of oxidant production, with the enhancing effect being slightly reduced in the knock-out mice. Using liver microsomes prepared from wild-type and knock-out mice as a source of CYP, Sch B was found to be a good co-substrate for the CYP-catalyzed reaction, with the rate of NADPH oxidation observable in microsomes prepared from knock-out mice being slower. The CYP-catalyzed reaction with Sch B was associated with a concomitant production of oxidant species, with the extent of oxidant production being reduced in cyp2e1 knock-out mouse microsomes. Taken together, the results indicate that CYP2E1 is partly responsible for the hepatic metabolism of Sch B that may trigger the antioxidant response in vivo.

Key words

schisandrin B cytochrome P-450 2E1 knock-out mitochondria glutathione heat shock proteins liver 





carbon tetrachloride


cytochrome P-450


dimethyl diphenyl bicarboxylate


glutathione antioxidant status


glutathione reductase


reduced glutathione


glutathione S-transferases


heat shock protein

Sch B

schisandrin B


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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Po Yee Chiu
    • 1
  • Hoi Yan Leung
    • 1
  • Michel K. T. Poon
    • 1
  • Susanna S. T. Lee
    • 2
  • Kam Ming Ko
    • 1
  1. 1.Department of BiochemistryThe Hong Kong University of Science & TechnologyChina
  2. 2.Department of BiochemistryThe Chinese University of Hong KongChina

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