Molecular and Cellular Biochemistry

, Volume 286, Issue 1–2, pp 161–170 | Cite as

Identification and characterization of recombinant and native rat myristoyl-CoA: protein N-myristoyltransferases

  • Vincent Rioux
  • Erwan Beauchamp
  • Frédérique Pedrono
  • Stéphanie Daval
  • Daniel Molle
  • Daniel Catheline
  • Philippe Legrand
Article

Abstract

Compared to other species that possess a single functional myristoyl-CoA: protein N-myristoyltransferase gene copy, human, mouse and cow possess 2 NMT genes, and more than 2 protein isoforms. In mammals, the contribution of each gene transcript to multiple protein isoform expression and enzyme activity remains unclear. In order to get new insight on their respective physiological role, we have cloned and characterized the two rat NMT cDNAs. Rat NMT1 and NMT2 cDNAs contain 1491 and 1590 nucleotides, respectively, with high identity with their mouse homologues. Polypeptide sequences exhibited 68.1% identity between NMT1 and 2. Recombinant rat NMT1 and 2 showed major immunoreactive forms at 66 and 50 kDa, although NMT2 is 33-amino acid longer than NMT1. Both proteins exhibited functional myristoyltransferase activity but NMT2 appeared to be 4-time less active than NMT1. Studies of native protein expression revealed that the level and sizes of NMT proteins greatly vary among rat tissues although NMT1 and 2 did not display tissue specific expression at the mRNA level. Altogether, these results suggest that NMT2 may contribute little to total NMT activity levels in vivo.

Keywords

NMT N-terminal myristoylation myristic acid saturated fatty acid metabolism rat 

Abbreviations:

NMT

myristoyl-CoA: protein N-myristoyltransferase

hNMT

human NMT

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Vincent Rioux
    • 1
  • Erwan Beauchamp
    • 1
  • Frédérique Pedrono
    • 1
  • Stéphanie Daval
    • 1
  • Daniel Molle
    • 2
  • Daniel Catheline
    • 1
  • Philippe Legrand
    • 1
    • 3
  1. 1.Laboratoire de BiochimieINRA-AgrocampusRennesFrance
  2. 2.UMR STLOINRA-AgrocampusRennesFrance
  3. 3.Laboratoire de BiochimieINRA-AgrocampusRENNES CedexFRANCE

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