Molecular and Cellular Biochemistry

, Volume 287, Issue 1–2, pp 109–116

Quercetin downregulates matrix metalloproteinases 2 and 9 proteins expression in prostate cancer cells (PC-3)

  • M. R. Vijayababu
  • A. Arunkumar
  • P. Kanagaraj
  • P. Venkataraman
  • G. Krishnamoorthy
  • J. Arunakaran
Article

DOI: 10.1007/s11010-005-9085-3

Cite this article as:
Vijayababu, M.R., Arunkumar, A., Kanagaraj, P. et al. Mol Cell Biochem (2006) 287: 109. doi:10.1007/s11010-005-9085-3

Abstract

Background: Cancer metastasis, involving multiple processes and various cytophysiological changes, is a primary cause of cancer death and may complicate the clinical management, even lead to death. Quercetin is a flavonoid and widely used as an antioxidant and recent studies have revealed its pleiotropic anticancer and antiproliferative capabilities. Gelatinases A and B (matrixmetalloproteinases 2 and 9) are enzymes known to involve in tumor invasion and metastases. In this study, we observed the precise involvement of quercetin role on these proteinases expression and activity. Design and methods: PC-3 cells were treated with quercetin at various concentrations (50 and 100 μM), for 24 h period and then subjected to western blot analysis to investigate the impact of quercetin on matrix metalloproteinase-2 (MMP-2) and 9 (MMP-9) expressions. Conditioned medium and cell lysate of quercetin-treated PC-3 cells were subjected to western blot analysis for proteins expression of MMP-2 and MMP-9. Gelatin zymography was also performed in quercetin treated PC-3 cells. Results: The results showed that quercetin treatment decreased the expressions of MMP-2 and MMP-9 in dose-dependent manner. The level of pro-MMP-9 was found to be high in the 100 μM quercetin-treated cell lysate of PC-3 cells, suggesting inhibitory role of quercetin on pro-MMP-9 activation. Gelatin zymography study also showed the decreased activities of MMP-2 and MMP-9 in quercetin treated cells. Conclusion: Hence, we speculated that inhibition of metastasis-specific MMPs in cancer cells may be one of the targets for anticancer function of quercetin, and thus provides the molecular basis for the development of quercetin as a novel chemopreventive agent for metastatic prostate cancer.

Key words

prostate cancer quercetin matrixmetalloproteinases metastases PC-3 cells 

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • M. R. Vijayababu
    • 1
  • A. Arunkumar
    • 1
  • P. Kanagaraj
    • 1
  • P. Venkataraman
    • 1
  • G. Krishnamoorthy
    • 1
  • J. Arunakaran
    • 1
  1. 1.Department of Endocrinology, Dr. ALM Postgraduate Institute of Basic Medical SciencesUniversity of MadrasChennaiIndia

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