Molecular and Cellular Biochemistry

, Volume 285, Issue 1–2, pp 29–34 | Cite as

Lipids and DNA oxidation in Staphylococcus aureus as a consequence of oxidative stress generated by ciprofloxacin

  • María Cecilia Becerra
  • Paulina Laura Páez
  • Laura E. Laróvere
  • Inés Albesa


Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to detect the formation of the amplified intermediate between reactive species oxygen and cytoplasmic macromolecules, namely malondialdehyde (MDA). The sensitive strain presented higher peroxidation of lipids than the resistant strain. The oxidative consequence for DNA was investigated by means of bacteria incubation with ciprofloxacin and posterior extraction of DNA, which was studied by high performance liquid chromatography (HPLC). Sensitive S. aureus ATCC 29213 showed an increase of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) respect controls without antibiotic; there was evident increase of the ratio between 8-oxodG and deoxyguanosine (dG) as a consequence of oxidation of dG to 8-oxodG considered the major DNA marker of oxidative stress. The resistant strain showed low oxidation of DNA and the analysis of 8-oxodG/dG ratio indicated lesser formation of 8-oxodG than S. aureus ATCC 29213.

Key words

ciprofloxacin DNA lipids oxidative stress Staphylococcus aureus 


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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • María Cecilia Becerra
    • 1
  • Paulina Laura Páez
    • 1
  • Laura E. Laróvere
    • 2
  • Inés Albesa
    • 1
  1. 1.Departamento Farmacia, Facultad de Ciencias QuímicasUniversidad Nacional de CórdobaCórdobaArgentina
  2. 2.Centro de Estudio de las Metabolopatías Congénitas, Cátedra de Clínica Pediátrica, Facultad de Ciencias MédicasUniversidad Nacional de Córdoba, Hospital de Niños de la Santísima TrinidadCórdobaArgentina

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