Molecular and Cellular Biochemistry

, Volume 285, Issue 1–2, pp 29–34 | Cite as

Lipids and DNA oxidation in Staphylococcus aureus as a consequence of oxidative stress generated by ciprofloxacin

  • María Cecilia Becerra
  • Paulina Laura Páez
  • Laura E. Laróvere
  • Inés Albesa
Article

Abstract

Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to detect the formation of the amplified intermediate between reactive species oxygen and cytoplasmic macromolecules, namely malondialdehyde (MDA). The sensitive strain presented higher peroxidation of lipids than the resistant strain. The oxidative consequence for DNA was investigated by means of bacteria incubation with ciprofloxacin and posterior extraction of DNA, which was studied by high performance liquid chromatography (HPLC). Sensitive S. aureus ATCC 29213 showed an increase of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) respect controls without antibiotic; there was evident increase of the ratio between 8-oxodG and deoxyguanosine (dG) as a consequence of oxidation of dG to 8-oxodG considered the major DNA marker of oxidative stress. The resistant strain showed low oxidation of DNA and the analysis of 8-oxodG/dG ratio indicated lesser formation of 8-oxodG than S. aureus ATCC 29213.

Key words

ciprofloxacin DNA lipids oxidative stress Staphylococcus aureus 

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References

  1. 1.
    Cabiscol E, Tamarit J, Ros J: Oxidative stress in bacteria and protein damage by reactive oxygen species. Int Microbiol 3: 3–8, 2000PubMedGoogle Scholar
  2. 2.
    Yoon SJ, Park JE, Yang JH, Park JW: OxyR regulon controls lipid peroxidation-mediated oxidative stress in Escherichia coli. J Biochem Mol Biol 35: 297–301, 2002PubMedGoogle Scholar
  3. 3.
    Lee HS, Lee YS, Kim HS, Kim HS, Choi JY, Hassan HM, Chung MH: Mechanism of regulation of 8-hydroxyguanine endonuclease by oxidative stress: Roles of FNR, ArcA, and Fur. Free Radic Biol Med 24: 1193–201, 1998CrossRefGoogle Scholar
  4. 4.
    Johnson KA, Mierzwa ML, Fink SP, Marnett LJ: MutS recognition of exocyclic DNA adducts that are endogenous products of lipid oxidation. J Biol Chem 274: 27112–27118, 1999PubMedCrossRefGoogle Scholar
  5. 5.
    Kiley PJ, Storz G: Exploiting thiol modifications. PLoS Biol 2: e400, 2004PubMedCrossRefGoogle Scholar
  6. 6.
    Becerra MC, Albesa I: Oxidative stress induced by ciprofloxacin in Staphylococcus aureus. Biochem Biophys Res Commun 297: 1003–1007, 2002PubMedCrossRefGoogle Scholar
  7. 7.
    Gurbay A, Gonthier B, Daveloose D, Favier A, Hincal F: Microsomal metabolism of ciprofloxacin generates free radicals. Free Radic Biol Med 30: 1118–1121, 2001PubMedCrossRefGoogle Scholar
  8. 8.
    Spratt TE, Schultz SS, Levy DE, Chen D, Schluter G, Williams GM: Different mechanisms for the photoinduced production of oxidative DNA damage by fluoroquinolones differing in photostability. Chem Res Toxicol 12: 809–815, 1999PubMedCrossRefGoogle Scholar
  9. 9.
    Belvedere A, Bosca F, Catalfo A, et al.: Type II guanine oxidation photoinduced by the antibacterial fluoroquinolone Rufloxacin in isolated DNA and in 2′-deoxyguanosine. Chem Res Toxicol 15: 1142–1149, 2002PubMedCrossRefGoogle Scholar
  10. 10.
    Cadet J, Bellon S, Berger M, Cuquerella MC, de Guidi G, Miranda MA: Recent aspects of oxidative DNA damage: guanine lesions, measurement and substrate specificity of DNA repair glycosylases. Biol Chem 383: 933–943, 2002PubMedCrossRefGoogle Scholar
  11. 11.
    Albesa I, Becerra MC, Battan PC, Paez PL: Oxidative stress involved in the antibacterial action of different antibiotics. Biochem Biophys Res Commun 317: 605–609, 2004PubMedCrossRefGoogle Scholar
  12. 12.
    Barriere C, Centeno D, Lebert A, Leroy-Setrin S, Berdague JL, Talon R: Roles of superoxide dismutase and catalase of Staphylococcus xylosus in the inhibition of linoleic acid oxidation. FEMS Microbiol Lett 201: 181–185, 2001PubMedCrossRefGoogle Scholar
  13. 13.
    Lee MH, Park JW: Lipid peroxidation products mediate damage of superoxide dismutase. Biochem Mol Biol Int 35: 1093–1102, 1995Google Scholar
  14. 14.
    Plastaras JP, Riggins JN, Otteneder M, Marnett LJ: Reactivity and mutagenicity of endogenous DNA oxopropenylating agents: base propenals, malondialdehyde, and N(epsilon)-oxopropenyllysine. Chem Res Toxicol 13: 1235–1242, 2000PubMedCrossRefGoogle Scholar
  15. 15.
    Lhiaubet-Vallet V, Sarabia Z, Hernandez D, Castell JV, Miranda MA: In vitro studies on DNA-photosensitization by different drug stereoisomers. Toxicol In Vitro 17: 651–656, 2003PubMedCrossRefGoogle Scholar
  16. 16.
    Becerra MC, Eraso AJ, Albesa I: Comparison of oxidative stress induced by ciprofloxacin and pyoverdin in bacteria and in leukocytes to evaluate toxicity. Luminescence 18: 334–340, 2003PubMedCrossRefGoogle Scholar
  17. 17.
    Becerra MC, Sarmiento M, Paez PL, Arguello G, Albesa I: Light effect and reactive oxygen species in the action of ciprofloxacin on Staphylococcus aureus. J Photochem Photobiol B 76: 13–18, 2004PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • María Cecilia Becerra
    • 1
  • Paulina Laura Páez
    • 1
  • Laura E. Laróvere
    • 2
  • Inés Albesa
    • 1
  1. 1.Departamento Farmacia, Facultad de Ciencias QuímicasUniversidad Nacional de CórdobaCórdobaArgentina
  2. 2.Centro de Estudio de las Metabolopatías Congénitas, Cátedra de Clínica Pediátrica, Facultad de Ciencias MédicasUniversidad Nacional de Córdoba, Hospital de Niños de la Santísima TrinidadCórdobaArgentina

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