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Molecular and Cellular Biochemistry

, Volume 284, Issue 1–2, pp 183–188 | Cite as

Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene

  • Yoshiyuki Kusakari
  • Eisaku Ogawa
  • Yuji Owada
  • Noriko Kitanaka
  • Hiroshi Watanabe
  • Michiyo Kimura
  • Hachiro Tagami
  • Hisatake Kondo
  • Setsuya Aiba
  • Ryuhei OkuyamaEmail author
Article

Abstract

Fatty acids are shown to be important in various skin functions. Fatty acid binding protein (FABP) is postulated to serve as a lipid shuttle, solubilizing hydrophobic fatty acids and delivering them to the appropriate metabolic system. Among the FABP family proteins, epidermal-type FABP (E-FABP) is solely expressed in keratinocyte but its specific role in skin is not yet fully established. We found an elevated expression of E-FABP in regenerative keratinocytes of healing wounds. However, E-FABP null mice showed no marked differences compared to wild type mice in the process of wound closure, in vivo. On the other hand, in keratinocyte culture, E-FABP gene disruption decreased the cell motility, but did not affect the cell proliferation. E-FABP deletion may be compensated for in vivo by the microenvironment comprised of various cells such as fibroblasts and endothelial cells around the wound. Our analyses suggest that the E-FABP elevation may be necessary for the activation of cell motility within regenerative epidermis during wound healing.

Keywords

cell motility FABP fatty acid keratinocyte regeneration wound healing 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Yoshiyuki Kusakari
    • 1
  • Eisaku Ogawa
    • 1
  • Yuji Owada
    • 2
  • Noriko Kitanaka
    • 2
  • Hiroshi Watanabe
    • 1
  • Michiyo Kimura
    • 1
  • Hachiro Tagami
    • 1
  • Hisatake Kondo
    • 2
  • Setsuya Aiba
    • 1
  • Ryuhei Okuyama
    • 1
    • 3
    Email author
  1. 1.Department of DermatologyTohoku University Graduate School of MedicineTohokuJapan
  2. 2.Department of HistologyTohoku University Graduate School of MedicineTohokuJapan
  3. 3.Department of DermatologyTohoku University Graduate School of MedicineAoba-kuJapan

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