Molecular and Cellular Biochemistry

, Volume 277, Issue 1–2, pp 117–125

High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2)

  • Yunfeng Li
  • Yongsheng Chang
  • Lifeng Zhang
  • Qiping Feng
  • Zhuo Liu
  • Yongwei Zhang
  • Jin Zuo
  • Yan Meng
  • Fude Fang
Article

Abstract

A new Rattus norvegicus PanK gene was isolated by mRNA differential display from high concentration glucose-stimulated rat, which encodes a human PanK4-like protein with KOG2201 and KOG4584 domain. Proteins that interact with rat PanK4 were identified by the application of the yeast two-hybrid system. One of the components, Pkm2, was found to be associated with rat PanK4 and its two domains under both in vitro and in vivo conditions. Immunofluorescence staining and confocal scanning experiments showed that PanK4 could transiently co-express with Pkm2 in the cytoplasm of HeLa cell and HEK293T cell. These findings suggest that PanK4 interacts with Pkm2 and thereby may modulate the glucose metabolism through regulating the activity of Pkm2.

Keywords

blood glucose regulation yeast two-hybrid system glucose metabolism type 2 diabetes 

Abbreviation:

PanK

Pantothenate kinase

Pkm2

M2-type pyruvate kinase

aa

amino acid residues

GST

glutathione-S-transferase

PBS

phosphate-buffered saline

HEK

human embryonic kidney

MoAb

monoclonal antibody

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Yunfeng Li
    • 1
    • 3
  • Yongsheng Chang
    • 1
  • Lifeng Zhang
    • 1
  • Qiping Feng
    • 1
  • Zhuo Liu
    • 1
  • Yongwei Zhang
    • 1
  • Jin Zuo
    • 1
  • Yan Meng
    • 1
  • Fude Fang
    • 1
  1. 1.National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical SciencesBeijingP.R. China
  2. 2.Department of Biochemistry and Molecular BiologyMississippi State UniversityMississippi StateUSA
  3. 3.Department of Biological ChemistryUniversity of CaliforniaIrvineUSA

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