Molecular and Cellular Biochemistry

, Volume 276, Issue 1–2, pp 113–119 | Cite as

Changes in electric charge and phospholipids composition in human colorectal cancer cells

  • Izabela Dobrzyńska
  • Barbara Szachowicz-Petelska
  • Stanisław Sulkowski
  • Zbigniew Figaszewski
Article

Abstract

Cancer cells perform their malicious activities through own cell membranes that screen and transmit inhibitory and stimulatory signals out of the cells and into them. This work is focused on changes of phospholipids content (PI—phosphatidylinositol, PS—phosphatidylserine, PE—phosphatidylethanolamine, PC—phosphatidylcholine) and electric charge that occur in cell membranes of colorectal cancer of pT3 stage, various grades (G2, G3) and without/with metastasis. Qualitative and quantitative composition of phospholipids in the membrane was determined by HPLC (high-performance liquid chromatography). The surface charge density of colorectal cancer cell membranes was measured using electrophoresis. The measurements were carried out at various pH of solution. It was shown that the process of cancer transformation was accompanied by an increase in total amount of phospholipids as well as an increase in total positive charge at low pH and total negative charge at high pH. A malignant neoplasm cells with metastases are characterized by a higher PC/PE ratio than malignant neoplasm cells without metastases. (Mol Cell Biochem 276: 113–119, 2005)

Keywords

phospholipids electric charge colorectal cancer cells 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cullis PR, Hope MJ: Physical properties and functional roles of lipids in membranes. In: D.E. Vance, J. Vance (eds.) Biochemistry of Lipids, Lipoproteins and Membranes. Elsevier Science Publishers, Amsterdam, pp 1–41, 1991Google Scholar
  2. 2.
    Stoll BA, Secreto G: New hormone-related markers of high risk to breast cancer. Ann Oncol 3: 435–438, 1992PubMedGoogle Scholar
  3. 3.
    Ruiz-Cabello J, Cohen JS: Phospholipids metabolites as indicators of cancer cell function. NMR Biomed 5: 226–233, 1992PubMedGoogle Scholar
  4. 4.
    Skrzydlewska E, Sulkowska M, Koda M, Sulkowski S: Proteolytic-antiproteolytic balance and its regulation in carcinogenesis. World J Gastroenterol 11: 1251–1266, 2005PubMedGoogle Scholar
  5. 5.
    Koda M, Reszeć J, Sulkowska M, Kańczuga-Koda L, Sulkowski S: Expression of the insulin-like growth factor-I receptor and proapoptotic Bax and Bak proteins in human colorectal cancer. Ann NY Acad Sci 1039: 377–383, 2004CrossRefGoogle Scholar
  6. 6.
    Baldassarre G, Nicoloso MS, Schiappacassi M, Chimienti E, Belletti B: Linking inflammation to cell cycle progression. Curr Pharm Des 10: 1653–1666, 2004CrossRefPubMedGoogle Scholar
  7. 7.
    Tsunada S, Iwakiri R, Fujimoto K, Aw TY: Chronic lipid hydroperoxide stress suppresses mucosal proliferation in rat intestine: potentiation of ornithine decarboxylase activity by epidermal growth factor. Dig Dis Sci 48: 2333–2341, 2003CrossRefPubMedGoogle Scholar
  8. 8.
    Zou W, Borelli S, Gilbert M, Liu T, Pon RA, Jennings HJ: Bioengineering of surface GD3 ganglioside for immunotargeting human melanoma cells. J Biol Chem 279: 25390–25399, 2004CrossRefPubMedGoogle Scholar
  9. 9.
    Evans WH: Fractionation of liver plasma membranes prepared by zonal centrifugation. Biochem J 166: 833–842, 1970Google Scholar
  10. 10.
    Szachowicz-Petelska B, Dobrzyńska I, Figaszewski Z, Sulkowski S: Changes in physico-chemical properties of human large intestine tumour cells membrane. Mol Cell Biochem 238: 41–47, 2002CrossRefPubMedGoogle Scholar
  11. 11.
    Ostrowska J, Skrzydlewska E, Figaszewski Z: Isolation and analysis of phospholipids. Chem Anal 45: 613–618, 2000Google Scholar
  12. 12.
    Krysiński P, Tien HY: Membrane electrochemistry. Prog Surf Sci 23: 317–412, 1986CrossRefGoogle Scholar
  13. 13.
    Barrow GM: Physical Chemistry. McGraw-Hill, Inc., New York, 1996Google Scholar
  14. 14.
    Mountford CE, Wright LC: Organization of lipids in the plasma membranes of malignant and stimulated cells: a new model. Trends Biochem Sci 13: 172–177, 1988CrossRefPubMedGoogle Scholar
  15. 15.
    Dueck DA, Chan M, Tran K, Wong JT, Jay FT, Littman C, Stimpson R, Choy PC: The modulation of choline phosphoglyceride metabolism in human colon cancer. Mol Cell Biochem 162(2): 97–103, 1996CrossRefPubMedGoogle Scholar
  16. 16.
    Monteggia E, Colombo I, Guerra A, Berra B: Phospholipid distribution in murine mammary adenocarcinomas induced by activated neu oncogene. Cancer Detect Prev 24: 207–211, 2000PubMedGoogle Scholar
  17. 17.
    Jackowski S: Cell cycle regulation of membrane phospholipids metabolism. J Biol Chem 271: 20219–20222, 1996CrossRefPubMedGoogle Scholar
  18. 18.
    Jackowski S: Coordination of membrane phospholipids synthesis with the cell cycle. J Biol Chem 269: 3858–3867, 1994PubMedGoogle Scholar
  19. 19.
    Podo F: Tumour phospholipids metabolism. NMR Biomed 12: 413–439, 1999CrossRefPubMedGoogle Scholar
  20. 20.
    Bos R, van Diest PJ, van der Groep P, Shvarts A, Greijer AE, van der Wall E: Expression of hypoxia-inducible factor-1κ and cell cycle proteins in invasive breast cancer are estrogen receptor related. Breast Cancer Res 6: R450–R459, 2004CrossRefPubMedGoogle Scholar
  21. 21.
    Zhao J, Zhou Q, Wiedmer T, Sims PJ: Level of expression of phospholipid scramblase regulates induced movement of phosphatidylserine to the cell surface. J Biol Chem 273: 6603–6606, 1998CrossRefPubMedGoogle Scholar
  22. 22.
    Ran S, Downes A, Thorpe PE: Increased exposure of anionic phospholipids on the surface of tumor blood vessels. Cancer Res 62: 6132–6140, 2002PubMedGoogle Scholar
  23. 23.
    Jakielaszek JM, Madej JA, Sobiech KA: Glycoproteins and sialoproteins in the serum and internal organs of mice with transplantable leukemia. Pol Arch Weter 26: 95–105, 1986PubMedGoogle Scholar
  24. 24.
    Erbil KM, Sen SE, Zincke H, Jones JD: Significance of serum protein and lipid-bound sialic acid as a marker for genitourinary malignancies. Cancer 57: 1389–1394, 1986PubMedGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Izabela Dobrzyńska
    • 1
  • Barbara Szachowicz-Petelska
    • 1
  • Stanisław Sulkowski
    • 2
  • Zbigniew Figaszewski
    • 1
    • 3
  1. 1.Institute of ChemistryUniversity in BiałystokBiałystokPoland
  2. 2.Department of PathologyMedical University of BiałystokBiałystokPoland
  3. 3.Laboratory of Electrochemical Power Sources, Faculty of ChemistryUniversity of WarsawWarsawPoland

Personalised recommendations