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Molecular and Cellular Biochemistry

, Volume 271, Issue 1–2, pp 1–11 | Cite as

Anticarcinogenic effect of Nymphaea alba against oxidative damage, hyperproliferative response and renal carcinogenesis in Wistar rats

  • Naghma Khan
  • Sarwat SultanaEmail author
Article

Abstract

The present study investigates the prophylactic effect of Nymphaea alba against ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats. Treatment with Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) enhanced iron-ascorbate-induced renal lipid peroxidation, xanthine oxidase, γ-glutamyl transpeptidase and hydrogen peroxide (H2O2) generation with reduction in renal glutathione content, antioxidant enzymes, viz., glutathione peroxidase, glutathione reductase, catalase, glucose-6-phosphate dehydrogenase and phase-II metabolising enzymes such as glutathione-S-transferase and quinone reductase. It also elevated the levels of blood urea nitrogen, serum creatinine, ornithine decarboxylase (ODC) activity and thymidine [3H] incorporation into renal DNA. It also enhanced DEN-initiated renal carcinogenesis by increasing the percentage incidence of renal tumors. Treatment of rats orally with N. alba (100 and 200 mg/kg body weight) resulted in significant decrease in γ-glutamyl transpeptidase, lipid peroxidation, xanthine oxidase, H2O2 generation, blood urea nitrogen, serum creatinine, renal ODC activity, DNA synthesis (p ≤ 0.001) and incidence of tumors. Renal glutathione content (p ≤ 0.001), glutathione metabolizing enzymes (p ≤ 0.001) and antioxidant enzymes were also recovered to significant level (p ≤ 0.001). Thus, our results show that N. alba is a potent chemopreventive agent and suppresses Fe-NTA-induced oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats. (Mol Cell Biochem 271: 1–11, 2005)

Key words

chemoprevention ferric nitrilotriacetate hyperproliferative response Nymphaea alba oxidative stress renal carcinogenesis 

abbreviation

AC

adenocarcinoma

CC

cancerous cells

DC

dense chromatin

DCT

distal convoluted tubule

G

glomerulus

HC

hyperchromatism

LIC

leucocytic infiltatory cells

NC

necrosis

NT

necrotic tissue

PM

pools of mucin

PT

proximal convoluted tubule

TC

tumor cells

TE

tubular epithelium

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References

  1. 1.
    Aruoma OI: Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat Res 523–524: 9–20, 2003Google Scholar
  2. 2.
    Block G: A role for antioxidants in reducing cancer risk. Nutr Rev 50: 207–213, 1992Google Scholar
  3. 3.
    Smith AG, Francis JE, Cathew P: Iron as a synergist for hepatocellular carcinoma induced by polychlorinated biphenyls in Ah-responsive C57BL/10ScSn mice. Carcinognesis 11: 437–444, 1990Google Scholar
  4. 4.
    Inoue S, Kawanishi S: Hydroxyl radical production and human DNA damage induced by ferric nitrilotriacetate and hydrogen peroxide. Cancer Res 47: 6522–6527, 1987Google Scholar
  5. 5.
    Anderson RL, Bishop WE, Campbell RL: A review of the environmental and mammalian toxicology of nitrilotriacetic acid. Crit Rev Toxicol 15: 1–102, 1985Google Scholar
  6. 6.
    Kawabata T, Ma Y, Yamador I, Okada S: Iron induced apoptosis in mouse renal proximal tubules after an injection of renal carcinogen, iron nitrilotriacetate. Carcinogenesis 18: 1389–1394, 1997Google Scholar
  7. 7.
    Hamazaki S, Okada S, Li JL: Oxygen reduction and lipid peroxidation by iron chelates with special reference to ferric nitrilotriacetate. Arch Biochem Biophys 272: 10–17, 1989Google Scholar
  8. 8.
    Tsao B, Curthoys NP: The absolute asymmetry of orientation of gamma glutamyl transpeptidase and amino-peptidase on the external surface of the rat renal brush border membrane. J Biol Chem 255: 7708–7711, 1980Google Scholar
  9. 9.
    Toyokuni S, Uchida K, Okamoto K, Hattori-Nakakuki Y, Hiai H, Stadtman ER: Formation of 4-hydroxy-2-nonenal modified proteins in the renal proximal tubules of rats treated with a renal carcinogen, ferric nitrilotriacetate. Proc Natl Acad Sci USA 91(7): 2616–2620, 1994Google Scholar
  10. 10.
    Khan N, Sharma S, Alam A, Saleem S, Sultana S: Tephrosia purpurea ameliorates N-diethylnitrosamine and potassium bromate-mediated renal oxidative stress and toxicity in Wistar rats. Pharmacol Toxicol 88: 294–299, 2001Google Scholar
  11. 11.
    Khan N, Sharma S, Sultana S: Nigella sativa ameliorates potassium bromate induced early events of carcinogenesis: Diminution of oxidative stress. Hum Exp Toxicol 22(4): 193–203, 2003Google Scholar
  12. 12.
    Khan N, Sharma S, Sultana S: Attenuation of potassium bromate-induced nephrotoxicity by coumarin (1,2-benzopyrone) in Wistar rats: Chemoprevention against free radical-mediated renal oxidative stress and tumor promotion response. Redox Rep 9(1): 19–28, 2004Google Scholar
  13. 13.
    Bhattacherjee SK: Handbook of Indian Medicinal Plants. Pointer Publishers, Jaipur, India, p. 22, 1998Google Scholar
  14. 14.
    Antonio A, Souza A, Brito RM: Oral anti-inflammatory and anti-ulcerogenic activities of a hydroalcoholic extract and partitioned fraction of Turnera ulmifolia (Turneaceae). J Ethnopharmacol 61: 215–228, 1988Google Scholar
  15. 15.
    Didry N, Duberwil L, Tratin F, Pinkas M: Antimicrobial activity of aerial parts of Drossera pelitata Smith on oral bacteria. J Ethnopharmacol 60: 215–228, 1988Google Scholar
  16. 16.
    Awai M, Narasaki M, Yamanoi Y, Seno S: Induction of diabetes in animals by parenteral administration of ferric nitrilotriacetate. A model of experimental hemochromatosis. Am J Pathol 95: 663–672, 1979Google Scholar
  17. 17.
    Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR: Bromobenzene induced liver necrosis: Protective role of glutathione and evidence for 3,4-bromobezene oxide as the hepatotoxic metabolite. Pharmacol 11: 151, 1974Google Scholar
  18. 18.
    Wright JR, Colby HD, Miles PR: Cytosolic factors that affect microsomal lipid peroxidation in lung and liver. Arch Biochem Biophys 206: 296–304, 1981Google Scholar
  19. 19.
    Kanter MW: Clinical Chemistry. The Bobber Merill Company Inc., USA, p. 80, 1975Google Scholar
  20. 20.
    Hare RS: Endogenous creatinine in serum and urine. Proc Soc Exp Biol Med 74: 148, 1950Google Scholar
  21. 21.
    Pick A, Keisari Y: Superoxide anion and H2O2 production by chemically elicited peritoneal macrophages-induction by multiple non-phagocytic stimulus. Cell Immunol 59: 301–308, 1981Google Scholar
  22. 22.
    Habig WH, Pabst MJ, Jakoby WB: Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem 249: 7130–7139, 1974Google Scholar
  23. 23.
    Carlberg I, Mannervik B: Glutathione level in rat brain. J Biol Chem 250: 4575–4480, 1975Google Scholar
  24. 24.
    Orlowski M, Meister A: γ-Glutamyl cyclotransferase distribution, isozymic forms and specificity. J Biol Chem 248: 2836–2844, 1973Google Scholar
  25. 25.
    Claiborne A: Catalase activity. In: R.A. Greenwald (ed). CRC Handbook of Methods in Oxygen Radical Research. CRC Press, Boca Raton, pp.~283–284, 1985Google Scholar
  26. 26.
    Mohandas M, Marshal JJ, Duggin GG, Horvath JS, Tiller D: Differential distribution of glutathione and glutathione related enzymes in rabbit kidney. Cancer Res 44: 5086–5091, 1984Google Scholar
  27. 27.
    Zaheer N, Tiwari KK, Krishnan PS: Exposure and solubilization of hepatic mitochondrial shunt dehydrogenases. Arch Biochem Biophys 109: 646–648, 1965Google Scholar
  28. 28.
    Athar M, Sharma SD, Iqbal M, Sultana S, Pandaya KB, Tripathi IP: Coordination of copper polyamines complex with immidiozoles potentiates its superoxide dismutase mimicking activity and abolishes its interactions with albumin. Biochem Mol Biol Int 39: 813–821, 1996Google Scholar
  29. 29.
    Benson AM, Hunkalar MJ, Talalay P: Increase of NADPH, quinone reductase activity by dietary antioxidant: Possible role in protection against carcinogenesis and toxicity. Proc Natl Acad Sci USA 77: 5116–5220, 1980Google Scholar
  30. 30.
    O’Brien TG, Simsiman RC, Boutwell RK: Induction of the polyamine biosynthetic enzymes in mouse epidermis by tumor promoting agents. Cancer Res 35: 1662–1670, 1975Google Scholar
  31. 31.
    Smart RC, Huang MT, Conney AH: Sn 1,2-diacylglycerols mimic the effects of TPA in vivo by inducing biochemical changes associated with tumor promotion in mouse epidermis. Carcinogenesis 7: 1865–1870, 1986Google Scholar
  32. 32.
    Giles KW, Myers A: An improved diphenyl method for the estimation of deoxyribonucleic acid. Nature 206: 93, 1965Google Scholar
  33. 33.
    Lowry OH, Rosebrough NJ, Farr A, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951PubMedGoogle Scholar
  34. 34.
    Wattenberg LW: An overview of chemoprevention: Current status and future prospects. Proc Soc Exp Biol Med 216: 133–141, 1997Google Scholar
  35. 35.
    Chesson A, Collins A: Assessment of the role of diet in cancer prevention. Cancer Lett 237–245, 1997Google Scholar
  36. 36.
    Cerutti PA: Prooxidant states and tumor promotion. Science 227: 375–381, 1985PubMedGoogle Scholar
  37. 37.
    Jambor J, Skrzypczak L: Flavonoids from the flowers of Nymphaea alba L. Acta Societatis Botanicorum Polaniae 60(1–2): 119–125, 1991Google Scholar
  38. 38.
    Jambor J, Skrzypczak L: Phenolic acids from the flowers of Nymphaea alba L. Acta Societatis Botanicorum Polania 60(1–2): 127–132, 1991Google Scholar
  39. 39.
    Joshi V, Merchant JR, Nadkarny VV, Namboori KK, Vaghani DD: Chemical constituents of some Indian medicinal plants. Indian J Chem 12(2): 226, 1974Google Scholar
  40. 40.
    Recio MC, Rios JL, Villar A: A review of some antimicrobial compounds isolated from medicinal plants reported in the literature 1978–1988. Phytoth Res 3(4): 117–125, 1989Google Scholar
  41. 41.
    Akagi K, Hirose M, Hoshiya T, Mizoguchi Y, Ito N, Shirai T: Modulating effects of ellagic acid, vanillin and quercetin in a rat medium term multi-organ carcinogenesis model. Cancer Lett 94(1): 113–121, 1995Google Scholar
  42. 42.
    Nishino H, Naitoh E, Iwashima A, Umezawa K: Quercetin interacts with calmodulin, A calcium regulating protein. Experimentia 40: 184–188, 1984Google Scholar
  43. 43.
    De Flora S, Ramel C: Mechanism of inhibition of mutagenesis and carcinogenesis: Classification and overview. Mutat Res 202: 285–306, 1988Google Scholar
  44. 44.
    Pegg AE, Shantz LM, Colemn CS: Ornithine decarboxylase as a target for chemoprevention. J Biol Chem 22: 132–138, 1995Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  1. 1.Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia HamdardHamdard UniversityNew DelhiIndia

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