Molecular Biology

, Volume 39, Issue 4, pp 593–607 | Cite as

NotI Sequence-Tagged Sites as Markers of Genes on Human Chromosome 3

  • G. E. Sulimova
  • E. R. Rakhmanaliev
  • E. A. Klimov
  • A. A. Kompaniytsev
  • I. G. Udina
  • E. R. Zabarovsky
  • L. L. Kisselev
Review and Experimantal Articles

Abstract

Using nucleotide sequences from jumping and linking NotI libraries of human chromosome 3, 94 NotI-STS markers for 72 individual NotI clones were developed. The positions of the NotI-STS markers and their order on the chromosome were determined by a combination of RH-mapping (our data), contig mapping, cytogenetic mapping, and in silico mapping. Comparison of NotI-STS DNAs with human genome sequences revealed two gaps in the regions 3p21.33 (marker NL1-256) and 3p21.31 (NL3-005), and a segmental duplication. Identical DNA fragments were found in the regions 12q and 3p22–21.33 (marker NL3-007). In the 3q28–q29 region (marker NLM-084), a fragment was detected whose identical copies were also present on chromosomes 1, 2, 15, and 19. For 69 NotI-STSs, significant homologies to nucleotide sequences of 70 genes and 2 cDNAs were detected (with homologies in NotI-STS 5′- and 3′-terminal sequences being taken into account). An association between NotI-STSs and genes is confirmed by a strong correlation between the density distributions of genes and NotI-STS markers on the map of human chromosome 3. Our results indicate that the NotI map may be regarded as a gene map of human chromosome 3. Thus, NotI-STSs are applicable as gene markers.

Key words

human chromosome 3 NotI clones NotI-STS markers PCR RH mapping physical mapping similarity to genes genomics 

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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • G. E. Sulimova
    • 1
  • E. R. Rakhmanaliev
    • 1
  • E. A. Klimov
    • 1
  • A. A. Kompaniytsev
    • 1
  • I. G. Udina
    • 1
  • E. R. Zabarovsky
    • 2
    • 3
  • L. L. Kisselev
    • 2
  1. 1.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia
  2. 2.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  3. 3.Microbiology and Tumor Biology Center and Center for Genomics and BioinformaticsKarolinska InstituteStockholmSweden

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