A Comprehensive Phylogenetic and Bioinformatics Assessment of Hydrophobin Protein (HYPAI) for Drug Delivery: an In Silico Analysis

  • H. Darsaraei
  • S. GhovvatiEmail author
  • S. A. Khodaparast


Nowadays, efforts are being made to reduce the side effects of drugs, increase the effectiveness of drugs on the target tissue, etc. using drug delivery knowledge to control the bioavailability of drugs in the human body. Hydrophobin is a low molecular weight hydrophobic fungal protein that can potentially be used as nanocarrier in drug delivery systems. Phylogenetic analysis showed that the hydrophobin of Trichoderma, Cladosporium, Verticillium and Sphaerulina species are closely related to animal hydrophobin. The evolutionary relationship of hydrophobin protein was investigated. The physiological and physicochemical properties of the HYPAI were studied by ProtScale and ProtParam servers. Post-translational modifications and three-dimensional structure of HYPAI were studied using CBS and Phyre2 servers, respectively. Protein–protein interactions were investigated using the platform of Search Tool for Retrieval of Interacting Genes and proteins (STRING). The results indicated that HYPAI is a highly hydrophobic, water soluble protein. The prediction of post-translational modification showed that this protein lacks a suitable site for glycosylation and mannosylation, but there was a possibility of phosphorylation in 8 sites. Physicochemical properties demonstrated that HYPAI is a stable protein with a half-life of 30 h in mammalian reticulocytes in vitro. In silico analysis of the interaction of HYPAI showed that no interaction has been reported for this protein in humans yet. The bioinformatics results have shown that HYPAI is a stable protein in vitro and has no immune effect in the human body, so that can pass through defense mechanisms of the human body with no immunogenicity reaction. Trichoderma spp., especially T. atroviride, due to its usage in agriculture and industries, can be a suitable candidate as a natural, reliable and safe bioreactor for the production of a cost-effective hydrophobin protein, and is being proposed for application in drug research as a new drug nanocarrier.


Drug delivery Hydrophobin Nanocarrier In silico analysis Bioinformatics analysis Phylogenetic 



It is sincerely appreciated by the Vice-Chancellor of Research and Technology of the University of Guilan, who provided the researchers with the necessary hardware and software facilities for the analyses carried out.

Compliance with Ethical Standards

Conflict of interest

We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.


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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Plant Pathology, Faculty of AgricultureUniversity of GuilanRashtIran
  2. 2.Department of Animal Science, Faculty of AgricultureUniversity of GuilanRashtIran

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