Design and Characterization of a Novel Hybrid Antimicrobial Peptide OM19R Based on Oncocin and MDAP-2

  • Lei Liu
  • Jie Liu
  • Qi Cui
  • Bo-Yan Jia
  • Zhi-Hua Pei
  • Kokou Ayefounin Odah
  • Yi-Ming Wang
  • Wen-Long Dong
  • Ling-Cong KongEmail author
  • Hong-Xia MaEmail author


Proline-rich antimicrobial peptides (PR-AMPs) have attracted much interest as they target Gram-negative and do not act by lytic mechanisms. In this study, a novel hybrid peptide OM19R was designed based on the Oncocin and MDAP-2. OM19R showed specific and improved potency against Escherichia coli (E. coli), Salmonella and Shigella standard strains compared to parental peptides. The minimum inhibitory concentrations (MICs) ranged from 1 to 2 μM. However, the activity against Klebsiella pneumoniae (K. pneumoniae) was inhibited. The dependence of the SbmA protein for OM19R activity was also studied by a gene-knockout strain. The confocal laser scanning microscopy further revealed that OM19R could rapidly penetrate into E. coli cells without membrane damage. In summary, the hybrid peptide OM19R is a strong cell-selective antibacterial peptide and a promising specific antibacterial agent.


Oncocin MDAP-2 PR-AMPs Gram-negative bacteria SbmA 



This work was supported by National Key Research and Development Plan (Grant Number: 2017YFD0501401), National Natural Science Foundation of China (Grant Numbers: 31572574, 31872519), Theme Guidance Project of Development of Science and Technology of Jilin Province (Grant Number: 20160204002NY), Natural Science Foundation of Jilin Province (Grant Number: 20180101003JC); The Education Department of Jilin province“13th Five Year Plan”science and technology research projects (JJKH20180829KJ).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

In this type of study, formal consent is not required.

Supplementary material

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Supplementary material 1 (DOCX 582 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyJilin Agricultural UniversityChangchunChina
  2. 2.School of Medical LaboratoryJilin Medical UniversityJilinChina
  3. 3.The Key Laboratory of Animal ProductionProduct Quality and Security, Ministry of Education, Jilin Agricultural UniversityChangchunChina

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