Exploring the Potentiality of Lactobacillus Cultures on the Production of Milk-Derived Bioactive Peptides with Antidiabetic Activity
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In the study, ten different Lactobacillus cultures were considered for the study of ɑ-amylase, ɑ-glucosidase and pancreatic lipase inhibitory activities. L. fermentum (M2) and L. fermentum (M7) were selected based on these three highest enzyme inhibition activities after 24 h at 37 °C inoculating at 2% rate. These two selected cultures were further studied for enzyme inhibition activities for different time intervals. For ɑ-amylase and ɑ-glucosidase inhibitory activities, M2 (65.29% and 11.14% respectively) and M7 (63.47% and 13.74% respectively) exhibited better inhibition after 24 h than other cultures. But in the case of lipase inhibitory activity, M7 (24.24%) and M2 (18.99%) showed maximum inhibition after 16 h. Purified peptides from fermented bovine milk were isolated, identified and characterized by amino acid profiling by searching on the BIOPEP database and Protein Information Resource (PIR) database. However, L. fermentum (M2) and L. fermentum (M7) could be explored to produce ɑ-amylase and ɑ-glucosidase inhibitory peptides from fermented bovine milk. Fermented bovine milk produced by selected Lactobacillus cultures could be a novel source of antidiabetic peptides.
KeywordsLactobacillus ɑ-Amylase inhibitory activity ɑ-Glucosidase inhibitory activity Lipase inhibitory activity Antidiabetic peptides
This research was financed by the Department of Biotechnology, New Delhi, Government of India (Grant No. Agri/2015/02).
Compliance with Ethical Standards
Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Aukrust TW, Brurberg MB, Nes IF (1995) Transformation of Lactobacillus by electroporation. In Electroporation protocols for microorganisms. Humana press, pp 201–208Google Scholar
- Axelsson L (2004) Lactic acid bacteria: classification and physiology. In: Salminen S, Wright AV, Ouwehand A (eds) Lactic acid bacteria: microbiological and functional aspects, 3rd edn. Marcel Dekker, New York, pp 1–67Google Scholar
- Carrasco-Castilla J, Hernández-Álvarez AJ, Jiménez-Martínez C, Jacinto-Hernández C, Alaiz M, Girón-Calle J, Vioque J, Dávila-Ortiz G (2012) Antioxidant and metal chelating activities of peptide fractions from phaseolin and bean protein hydrolysates. Food Chem 135(3):1789–1795PubMedCrossRefPubMedCentralGoogle Scholar
- Donkor O (2007) Influence of probiotic organisms on release of bioactive compounds in yoghurt and soy yoghurt. Doctoral dissertation, Victoria UniversityGoogle Scholar
- Elya B, Basah K, Mun'im A, Yuliastuti W, Bangun A, Septiana EK (2012) Screening of α-glucosidase inhibitory activity from some plants of Apocynaceae, Clusiaceae, Euphorbiaceae, and Rubiaceae. BioMed Res Int 2012:281078Google Scholar
- Mishra T, Goyal AK, Midha SK, Sen A (2011) Antioxidative properties of Canna edulis Ker-Gawl. Indian J Nat Prod Resour 2(3):315–321Google Scholar
- Moslehishad M, Ehsani MR, Salami M, Mirdamadi S, Ezzatpanah H, Naslaji AN, Moosavi-Movahedi AA (2013) The comparative assessment of ACE-inhibitory and antioxidant activities of peptide fractions obtained from fermented camel and bovine milk by Lactobacillus rhamnosus PTCC 1637. Int Dairy J 29(2):82–87CrossRefGoogle Scholar
- Mudgil P et al. (2016) Pancreatic lipase inhibitory activities of lactic acid bacteria isolated from raw camel milk. Conference Proceedings of IPC2016. Paper presented at the International Scientific Conference on Probiotics and Prebiotics, Budapest (p. 49). IPC2016Google Scholar
- Nair SS, Kavrekar V, Mishra A (2013) In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol 3(1):128–132Google Scholar
- Noriko N, Pambudi A (2014) Diversifikasi pangan sumber karbohidrat canna edulis Kerr.(Ganyong). Jurnal Al-Azhar Indonesia Seri Sains dan Teknologi 2(4):248–252Google Scholar
- Opatha Vithana NL (2012) A comparative study of immunomodulatory activity of deer and cow milk proteins. Doctoral dissertation, Lincoln UniversityGoogle Scholar
- Shai LJ, Magano SR, Lebelo SL, Mogale AM (2011) Inhibitory effects of five medicinal plants on rat alpha-glucosidase: comparison with their effects on yeast alpha-glucosidase. J Med Plants Res 5(13):2863–2867Google Scholar
- Yofananda O, Estiasih T (2016) Potensi senyawa bioaktif umbi-umbian lokal sebagai penurun kadar glukosa darah: kajian pustaka. Jurnal Pangan dan Agroindustri 4(1):410–416Google Scholar