Advertisement

Effects of Graded Inclusion of Bioactive Peptides Derived from Sesame Meal on the Growth Performance, Internal Organs, Gut Microbiota and Intestinal Morphology of Broiler Chickens

  • Mohammad Ebrahim Salavati
  • Vahid RezaeipourEmail author
  • Rohullah Abdullahpour
  • Naser Mousavi
Article
  • 1 Downloads

Abstract

The aim of this experiment was to evaluate the effects of bioactive peptides derived from sesame meal (BPSM) compared with mannan-oligosaccharides (MOS) as a prebiotic supplementation and avilamycin (as an antibiotic) on the productive performance, internal organs, gut microbial population, and intestinal morphology in broiler chickens. A total of 300 one-day- old broiler chicks were randomly allocated into 6 treatments with 5 replicates per treatment and 10 birds per replicate. The experimental treatments were a control diet or control diet supplemented with 50, 100 or 150 (mg/kg) BPSM or MOS (2 g/kg) and avilamycin (10 mg/kg). Growth performance traits, including daily weight gain, food intake and food conversion ratio (FCR) were recorded. At the end of the study, carcass characteristics, gut microbiot, and intestinal morphometric indices were determined. The results indicated that weight gain increased (P < 0.05) in birds received MOS and 100 mg/kg BPSM on days 1–11 and 1–32, respectively. The dietary treatments did not affect food consumption in broilers. However, FCR improved in broiler chickens fed 100 mg/kg BPSM supplement (P < 0.05). Inclusion of BPSM, MOS or antibiotic had no effect on the relative weight or length of internal organs compared to control group, except for gizzard weight on day 32. The relative weight of gizzard was significantly lower for MOS treatment than the control group (P < 0.05). Addition of antibiotic and 100 mg/kg BPSM supplementation increased the caecum population of Lactobacilli in broiler chickens (P < 0.05). Besides, diets supplemented with antibiotic, MOS or all graded levels of BPSM decreased the viable cell count of Escherichia coli in caecum segment of broiler chickens (P < 0.05). In the intestinal mrphometric indices, the villus length was greater in antibiotic, MOS, 100 or 150 mg/kg BPSM compared with control diet (P < 0.05). In addition, the birds fed diets supplemented with MOS had a greater crypt depth (P < 0.05). In conclusion, the positive effect of BPSM supplementation on the performance, gut microbiota, and intestinal morphology was clearly evident for broiler chickens.

Keywords

Bioactive peptides Intestinal morphology Microbiota Broilers 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors verify that they have no conflict of interest in this research.

Human and Animal Participants

The experiment was approved by the animal welfare commissioner of the Department of Animal Science, Islamic Azad University, Qaemshar branch (Qaemshahr, Iran).

Informed Consent

The manuscript does not contain any studies with human subjects performed by any of the authors.

References

  1. Abdollahi M, Zaefarian F, Gu Y, Xiao W, Jia J, Ravindran V (2017) Influence of soybean bioactive peptides on growth performance, nutrient utilisation, digestive tract development and intestinal histology in broilers. J Appl Anim Nutr 5Google Scholar
  2. Abdollahi M, Zaefarian F, Gu Y, Xiao W, Jia J, Ravindran V (2018) Influence of soybean bioactive peptides on performance, foot pad lesions and carcass characteristics in broilers. J Appl Anim Nutr 6Google Scholar
  3. Albenzio M, Santillo A, Caroprese M, della Malva A, Marino R (2017) Bioactive peptides in animal food products. Foods 6:35CrossRefGoogle Scholar
  4. Bandyopadhyay K, Ghosh S (2002) Preparation and characterization of papain-modified sesame (Sesamum indicum L.) protein isolates. J Agri Food Chem 50:6854–6857CrossRefGoogle Scholar
  5. Bao H et al (2009) Effects of pig antibacterial peptides on growth performance and intestine mucosal immune of broiler chickens. Poult Sci 88:291–297CrossRefGoogle Scholar
  6. Bhandari D, Rafiq S, Gat Y, Gat P, Waghmare R, Kumar V (2019) A Review on Bioactive Peptides: Physiological Functions, Bioavailability and Safety. Int J Pept Res Ther 1–12Google Scholar
  7. Biswas A, Dhar P, Ghosh S (2010) Antihyperlipidemic effect of sesame (Sesamum indicum L.) protein isolate in rats fed a normal and high cholesterol diet. J food Sci 75:H274–H279CrossRefGoogle Scholar
  8. Biswas A, Messam R, Kumawat M, Namit M, Mandal A, Mir N (2018) Effects of prebiotics on intestinal histo-morphometry and gut microflora status of broiler chickens. Indian J Anim Res 8:1–5Google Scholar
  9. Chand N, Faheem H, Khan RU, Qureshi MS, Alhidary IA, Abudabos AM (2016) Anticoccidial effect of mananoligosacharide against experimentally induced coccidiosis in broiler. Environ Sci Poll Res 23:14414–14421CrossRefGoogle Scholar
  10. Das R, Dutta A, Bhattacharjee C (2012) Preparation of sesame peptide and evaluation of antibacterial activity on typical pathogens. Food Chem 131:1504–1509CrossRefGoogle Scholar
  11. Eftekhari A, Rezaeipour V, Abdullahpour R (2015) Effects of acidified drinking water on performance, carcass, immune response, jejunum morphology, and microbiota activity of broiler chickens fed diets containing graded levels of threonine. Livest Sci 180:158–163CrossRefGoogle Scholar
  12. Fernandez F, Hinton M, Gils BV (2002) Dietary mannan-oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella Enteritidis colonization. Avian Pathol 31:49–58CrossRefGoogle Scholar
  13. Hajiaghapour M, Rezaeipour V (2018) Comparison of two herbal essential oils, probiotic, and mannan-oligosaccharides on egg production, hatchability, serum metabolites, intestinal morphology, and microbiota activity of quail breeders. Livest Sci 210:93–98CrossRefGoogle Scholar
  14. Hale JD, Hancock RE (2007) Alternative mechanisms of action of cationic antimicrobial peptides on bacteria. Exp Rev Anti Ther 5:951–959CrossRefGoogle Scholar
  15. Jahanian E, Mahdavi AH, Asgary S, Jahanian R (2016) Effect of dietary supplementation of mannanoligosaccharides on growth performance, ileal microbial counts, and jejunal morphology in broiler chicks exposed to aflatoxins. Livest Sci 190:123–130CrossRefGoogle Scholar
  16. Karimzadeh S, Rezaei M, Yansari A (2016) Effects of canola bioactive peptides on performance, digestive enzyme activities, nutrient digestibility, intestinal morphology and gut microflora in broiler chickens. Poult Sci J 4:27–36Google Scholar
  17. Karimzadeh S, Rezaei M, Yansari AT (2017) Effects of different levels of canola meal peptides on growth performance and blood metabolites in broiler chickens. Livest Sci 203:37–40CrossRefGoogle Scholar
  18. Kaur J, Kumar V, Sharma K, Kaur S, Gat Y, Goyal A, Tanwar B (2019) Opioid peptides: an overview of functional significance. Int J Pept Res Ther 1–9Google Scholar
  19. Liu B-L, Chiang P-S (2008) Production of hydrolysate with antioxidative activity and functional properties by enzymatic hydrolysis of defatted sesame (Sesamum indicum L.). Int J Appl Sci Eng 6:73–83Google Scholar
  20. Liu W, Cheng G, Liu H, Kong Y (2015) Purification and identification of a novel angiotensin I-converting enzyme inhibitory peptide from sesame meal. Int J Pept Res Ther 21:433–442CrossRefGoogle Scholar
  21. Marambe P, Wanasundara J (2012) Seed storage proteins as sources of bioactive peptides Bioactive molecules in plant foods Canada: Nova Science PublishersGoogle Scholar
  22. Mookiah S, Sieo CC, Ramasamy K, Abdullah N, Ho YW (2014) Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agri 94:341–348CrossRefGoogle Scholar
  23. Mourão JL et al (2006) Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Anim Feed Sci Technol 126:107–120CrossRefGoogle Scholar
  24. Nakano D et al (2006) Antihypertensive effect of angiotensin I-converting enzyme inhibitory peptides from a sesame protein hydrolysate in spontaneously hypertensive rats. Biosci Biotechnol Biochem 70:1118–1126CrossRefGoogle Scholar
  25. Osho S, Xiao W, Adeola O (2019) Response of broiler chickens to dietary soybean bioactive peptide and coccidia challenge. Poult Sci 98:1–10CrossRefGoogle Scholar
  26. Pelicano ERL, Souza P, Souza H, Figueiredo D, Boiago M, Carvalho S, Bordon V (2005) Intestinal mucosa development in broiler chickens fed natural growth promoters. Braz J Poult Sci 7:221–229CrossRefGoogle Scholar
  27. Pourabedin M, Xu Z, Baurhoo B, Chevaux E, Zhao X (2014) Effects of mannan oligosaccharide and virginiamycin on the cecal microbial community and intestinal morphology of chickens raised under suboptimal conditions. Can J Microbiol 60:255–266CrossRefGoogle Scholar
  28. Rezaeipour V, Barsalani A, Abdullahpour R (2016) Effects of phytase supplementation on growth performance, jejunum morphology, liver health, and serum metabolites of Japanese quails fed sesame (Sesamum indicum) meal-based diets containing graded levels of protein. Trop Anim Health Prod 48:1141–1146.  https://doi.org/10.1007/s11250-016-1066-x CrossRefPubMedGoogle Scholar
  29. SAS (1999) SAS Statistics user’s guide. Statistical analytical system (5th revised edn). SAS Institute Inc, Carry.Google Scholar
  30. Teng P-Y, Kim WK (2018) Roles of prebiotics in intestinal ecosystem of broilers. Front Vet Sci 5Google Scholar
  31. Wen L-F, He J-G (2012) Dose–response effects of an antimicrobial peptide, a cecropin hybrid, on growth performance, nutrient utilisation, bacterial counts in the digesta and intestinal morphology in broilers. Br J Nutr 108:1756–1763CrossRefGoogle Scholar
  32. Yamauchi K, Samanya M, Seki K, Ijiri N, Thongwittaya N (2006) Influence of dietary sesame meal level on histological alterations of the intestinal mucosa and growth performance of chickens. J Appl Poult Res 15:266–273CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Animal Science, Qaemshahr BranchIslamic Azad UniversityQaemshahrIran
  2. 2.Department of Animal Science, Varamin BranchIslamic Azad UniversityVaraminIran

Personalised recommendations