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Survey the Effect of Insulin on Modulating Feed Intake Via NPY Receptors in 5-Day-Old Chickens

  • Shiba Yousefvand
  • Farshid HamidiEmail author
  • Morteza Zendehdel
  • Abbas Parham
Article
  • 1 Downloads

Abstract

Some neuropeptides such as neuropeptide Y (NPY) maintain energy balance. The aim of this study was to investigate the role of insulin on feed intake and their interaction with NPY receptors (NPY1, NPY2, and NPY5) in newly hatched chickens (5-day-old chickens). This study was performed in 7 experiments (in each experiment, 4 groups with 6 replications). In the first 4 experiments were used to investigate the doses of insulin (2.5, 5 and 10 ng), NPY1, NPY2, and NPY5 receptors antagonist (1.25, 2.5 and 5 μg) on feed intake via intraventricular injection were evaluated, as well as effective and sub-effective doses were determined. After that, the interactions of these compounds with insulin were investigated in 3 experiments. In experiments 5, 6 and 7, chickens received, NPY1, NPY2, and NPY5 receptors antagonist, prior to injection of insulin, respectively; then the chicken was injected with insulin to study the feed intake. In each experiment, the control group received a control solution. Then, in each experiment cumulative feed intake were measured up to 120 min after injection. NPY2 receptor antagonist, resulted in increased feed intake (p < 0.01), while insulin, NPY1 and NPY5 receptors antagonist (p < 0.001) reduced feed intake. NPY1 receptor antagonist, amplificated reduced effect of insulin (p < 0.001), and NPY2 receptor antagonist (p < 0.01) demolished reduced effect of insulin on feed intake and increased it, but NPY5 receptor antagonist did not affect hypophagic effect of insulin (p > 0.05). It is concluded that, insulin has a decreasing effect on newly hatched chicken’s feed intake via NPY1 and NPY2 receptors.

Keywords

NPY receptor Insulin 5-day-old chickens Feed intake Intraventricular injection 

Notes

Acknowledgements

The authors thank the central laboratory (Dr. Rastegar Lab.) of the Faculty of Veterinary Medicine, University of Tehran for collaboration. This study was approved by license number 3/40151 at Ferdowsi University of Mashhad.

Compliance with Ethical Standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Research Involving Human and Animal Rights

All experiments were executed according to the Guide for the Care and Use of Laboratory Animals and were approved by the institutional animal ethics committee.

References

  1. Abolghasempour S, Zendehdel M, Panahi N, Jahandideh A, Gilanpour H (2018) Intracerebroventricular injection of the glutamatergic receptors antagonist affects N/OFQ-induced hyperphagia in neonatal broilers: role of NMDA and AMPA receptors. Int J Pept Res Ther.  https://doi.org/10.1007/s10989-018-9733-6 Google Scholar
  2. Batterham RL, Cowley MA, Small CJ, Herzog H, Cohen MA, Dakin CL, Wren AM, Brynes AE, Low MJ, Ghatei MA, Cone RD (2002) Gut hormone PYY 3-36 physiologically inhibits food intake. Nature 418(6898):650CrossRefGoogle Scholar
  3. Brome’e T, Sjödin P, Fredriksson R, Boswell T, Larsson T, Salaneck E, Zoorob R, Mohell N, Larhammar D (2006) Neuropeptide Y-family receptors Y6 and Y7 in chicken. The FEBS journal 273(9):2048–2063.  https://doi.org/10.1111/j.1742-4658.2006.05221.x CrossRefGoogle Scholar
  4. Carvalheira JB, Ribeiro EB, Araujo EP, Guimaraes RB, Telles MM, Torsoni M, Gontijo JA, Velloso LA, Saad MJ (2003) Selective impairment of insulin signalling in the hypothalamus of obese Zucker rats. Diabetologia 46(12):1629–1640.  https://doi.org/10.1007/s00125-003-1246-x CrossRefGoogle Scholar
  5. Carvalheira JB, Torsoni MA, Ueno M, Amaral ME, Araújo EP, Velloso LA, Gontijo JA, Saad MJ (2005) Cross-talk between the insulin and leptin signaling systems in rat hypothalamus. Obesity 13(1):48–57.  https://doi.org/10.1038/oby.2005.7 CrossRefGoogle Scholar
  6. Corp ES, McQuade J, Krasnicki S, Conze DB (2001) Feeding after fourth ventricular administration of neuropeptide Y receptor agonists in rats. Peptides 22(3):493–499CrossRefGoogle Scholar
  7. Daniels AJ, Grizzle MK, Wiard RP, Matthews JE, Heyer D (2002) Food intake inhibition and reduction in body weight gain in lean and obese rodents treated with GW438014A, a potent and selective NPY-Y5 receptor antagonist. Regul Pept 106(1–3):47–54.  https://doi.org/10.1016/S0167-0115(02)00034-4 CrossRefGoogle Scholar
  8. Davis JL, Masuoka DT, Gerbrandt LK, Cherkin A (1979) Auto radiographic distribution of L-proline in chicks after intracerebral injection. Physiol Behav 22(4):693–695.  https://doi.org/10.1016/0031-9384(79)90233-6 CrossRefGoogle Scholar
  9. Denbow DM, Van Krey HP, Lacy MP, Dietrick TJ (1983) Feeding, drinking and body temperature of leghorn chicks: effects of ICV injections of biogenic amines. Physiol Behav 31(1):85–90.  https://doi.org/10.1016/0031-9384 CrossRefGoogle Scholar
  10. Duarte AI, Moreira PI, Oliveira CR (2012) Insulin in central nervous system: more than just a peripheral hormone. J Aging Res 2012:1–21CrossRefGoogle Scholar
  11. Duhault J, Boulanger M, Chamorro S, Boutin JA, Zuana OD, Douillet E, Fauchère JL, Félétou M, Germain M, Husson B, Vega AM (2000) Food intake regulation in rodents: Y5 or Y1 NPY receptors or both? Can J Physiol Pharmacol 78(2):173–185.  https://doi.org/10.1139/y99-131 CrossRefGoogle Scholar
  12. Furuse M (2002) Central regulation of food intake in the neonatal chick. Animal Sci J 73(2):83–94.  https://doi.org/10.1046/j.1344-3941.2002.00014.x CrossRefGoogle Scholar
  13. Furuse M, Ando R, Bungo T, Shimojo M, Masuda Y (1999) Intracerebroventricular injection of orexins does not stimulate food intake in neonatal chicks. Br Poult Sci 40(5):698–700CrossRefGoogle Scholar
  14. Hamidi F, Yousefvand S (2017) Role of the hypothalamic arcuate nucleus in regulation of food intake (review study). J Neyshabur Univ Med Sci 5(1):52–65 (Persian) Google Scholar
  15. Honda K, Kamisoyama H, Saneyasu T, Sugahara K, Hasegawa S (2007) Central administration of insulin suppresses food intake in chicks. Neurosci Lett 423(2):153–157CrossRefGoogle Scholar
  16. Hussain SS, Bloom SR (2013) The regulation of food intake by the gut-brain axis: implications for obesity. Int J Obes 37(5):625–633.  https://doi.org/10.1038/ijo.2012.93 CrossRefGoogle Scholar
  17. Jalali M, Zendehdel M, Babapour V, Gilanpour H (2019) Interaction between central oxytocinergic and glutamatergic systems on food intake in neonatal chicks: role of NMDA and AMPA receptors. Int J Pept Res Ther 25(1):195–203.  https://doi.org/10.1007/s10989-017-9664-7 CrossRefGoogle Scholar
  18. Jensen J (2001) Regulatory peptides and control of food intake in non-mammalian vertebrates. Comp Biochem Physiol A Mol Integr Physiol 128(3):469–477.  https://doi.org/10.1016/S1095-6433(00)00329-9 CrossRefGoogle Scholar
  19. Joost HG (2012) Appetite control. Springer, BerlinCrossRefGoogle Scholar
  20. Kageyama H, Takenoya F, Hirako S, Wada N, Kintaka Y, Inoue S, Ota E, Ogawa T, Shioda S (2012) Neuronal circuits involving neuropeptide Y in hypothalamic arcuate nucleus-mediated feeding regulation. Neuropeptides 46(6):285–289.  https://doi.org/10.1016/jnpep.2012.09.007 CrossRefGoogle Scholar
  21. Kanatani A, Mashiko S, Murai N, Sugimoto N, Ito J, Fukuroda T, Fukami T, Morin N, MacNeil DJ, Van der Ploeg LH, Saga Y (2000) Role of the Y1 receptor in the regulation of neuropeptide Y-mediated feeding: comparison of wild-type, Y1 receptor-deficient, and Y5 receptor-deficient mice. Endocrinology 141(3):1011–1016.  https://doi.org/10.1210/endo.141.3.7387 CrossRefGoogle Scholar
  22. Keen-Rhinehart E, Bartness TJ (2007) NPY Y1 receptor is involved in ghrelin-and fasting-induced increases in foraging, food hoarding, and food intake. Am J Physiol Regul Integr Comp Physiol 292(4):1728–1737.  https://doi.org/10.1152/ajpregu.00597.2006 CrossRefGoogle Scholar
  23. Levens NR, Feletou M, Galizzi JP, Fauchere JL, Della-Zuana O, Lonchampt M (2004) NPY effects on food intake and metabolism. In: Michel MC (ed) Neuropeptide Y and related peptides. Springer, Berlin, pp 285–315Google Scholar
  24. Levin BE, Israel P, Lattemann DF (1998) Insulin selectively down regulates α2-adrenoceptors in the arcuate and dorsomedial nucleus. Brain Res Bull 45(2):179–181.  https://doi.org/10.1016/S0361-9230(97)00336-5 CrossRefGoogle Scholar
  25. Loh K, Zhang L, Brandon A, Wang Q, Begg D, Qi Y, Fu M, Kulkarni R, Teo J, Baldock P, Brüning JC (2017) Insulin controls food intake and energy balance via NPY neurons. Mol Metab 6(6):574–584.  https://doi.org/10.1016/j.molmet.2017.03.013 CrossRefGoogle Scholar
  26. Loyd AN, Long CR, Lewis AW, Randel RD (2011) Effects of physiological age on residual feed intake of growing heifers. Open J Anim Sci 1(03):89CrossRefGoogle Scholar
  27. Lundell I, Boswell T, Larhammar D (2002) Chicken neuropeptide Y-family receptor Y4: a receptor with equal affinity for pancreatic polypeptide, neuropeptide Y and peptide YY. J Mol Endocrinol 28(3):225–235CrossRefGoogle Scholar
  28. Obici S, Feng Z, Karkanias G, Baskin DG, Rossetti L (2002) Decreasing hypothalamic insulin receptors causes hyperphagia and insulin resistance in rats. Nat Neurosci 5(6):566–572.  https://doi.org/10.1038/nn861 CrossRefGoogle Scholar
  29. Olanrewaju HA, Thaxton JP, Dozier WA, Purswell J, Roush WB, Branton SL (2006) A review of lighting programs for broiler production. Int J Poult Sci 5(4):301–308CrossRefGoogle Scholar
  30. Ortiz AA, Milardo LF, DeCarr LB, Buckholz TM, Mays MR, Claus TH, Livingston JN, Mahle CD, Lumb KJ (2007) A novel long-acting selective neuropeptide Y2 receptor polyethylene glycol-conjugated peptide agonist reduces food intake and body weight and improves glucose metabolism in rodents. J Pharmacol Exp Ther 323(2):692–700.  https://doi.org/10.1124/jpet.107.125211 CrossRefGoogle Scholar
  31. Plum L, Belgardt BF, Brüning JC (2006) Central insulin action in energy and glucose homeostasis. J Clin Invest 116(7):1761–1766.  https://doi.org/10.1172/JCI29063 CrossRefGoogle Scholar
  32. Salisu IB, Iyeghe-Erakpotobor GT (2014) Effect of age and sex of rabbit on nutrient intake and digestibility. J Global Biosci 3(3):569–575Google Scholar
  33. Shiraishi JI, Yanagita K, Fujita M, Bungo T (2008) Central insulin suppresses feeding behavior via melanocortins in chicks. Domest Anim Endocrinol 34(3):223–228CrossRefGoogle Scholar
  34. Shiraishi JI, Yanagita K, Nishikawa F, Tahara Y, Fujita M, McMurtry JP, Bungo T (2009) A comparison of the anorexic effects of chicken, porcine, human and bovine insulin on the central nervous system of chicks. J Poult Sci 46(2):144–148CrossRefGoogle Scholar
  35. Shiraishi JI, Tanizawa H, Fujita M, Kawakami SI, Bungo T (2011a) Localization of hypothalamic insulin receptor in neonatal chicks: evidence for insulinergic system control of feeding behavior. Neurosci Lett 491(3):177–180.  https://doi.org/10.1016/j.neulet.2011.01.031 CrossRefGoogle Scholar
  36. Shiraishi JI, Yanagita K, Fukumori R, Sugino T, Fujita M, Kawakami SI, McMurtry JP, Bungo T (2011b) Comparisons of insulin related parameters in commercial-type chicks: evidence for insulin resistance in broiler chicks. Physiol Behav 103(2):233–239CrossRefGoogle Scholar
  37. Silverstein JT, Plisetskaya EM (2000) The effects of NPY and insulin on food intake regulation in fish. Am Zool 40(2):296–308.  https://doi.org/10.1093/icb/40.2.296 Google Scholar
  38. Stanley BG, Magdalin W, Seirafi A, Thomas WJ, Leibowitz SF (1993) The perifornical area: the major focus of (a) patchily distributed hypothalamic neuropeptide Y-sensitive feeding system (s). Brain Res 604(1–2):304–317.  https://doi.org/10.1016/0006-8993(93)90382-W CrossRefGoogle Scholar
  39. Steculorum SM, Ruud J, Karakasilioti I, Backes H, Ruud LE, Timper K, Hess ME, Tsaousidou E, Mauer J, Vogt MC, Paeger L (2016) AgRP neurons control systemic insulin sensitivity via myostatin expression in brown adipose tissue. Cell 165(1):125–138.  https://doi.org/10.1016/j.cell.2016.02.044 CrossRefGoogle Scholar
  40. Sun QQ, Baraban SC, Prince DA, Huguenard JR (2003) Target-specific neuropeptide Y-ergic synaptic inhibition and its network consequences within the mammalian thalamus. J Neurosci 23(29):9639–9649CrossRefGoogle Scholar
  41. Wynne K, Stanley S, McGowan B, Bloom S (2005) Appetite control. J Endocrinol 184(2):291–318.  https://doi.org/10.1677/joe.1.05866 CrossRefGoogle Scholar
  42. Yousefvand S, Hamidi F, Zendehdel M, Parham A (2018a) Hypophagic effects of insulin is mediated via NPY1/NPY2 receptors in broiler cockerels. Can J Physiol Pharmacol 96(12):1301–1307.  https://doi.org/10.1139/cjpp-2018-0470 CrossRefGoogle Scholar
  43. Yousefvand S, Hamidi F, Zendehdel M, Parham A (2018b) Interaction of neuropeptide Y receptors (NPY1, NPY2 and NPY5) with somatostatin on somatostatin-induced feeding behaviour in broiler chicken. Br Poult Sci 60(1):71–78.  https://doi.org/10.1080/00071668.2018.1547359 CrossRefGoogle Scholar
  44. Yulyaningsih E, Loh K, Lin S, Lau J, Zhang L, Shi Y, Berning BA, Enriquez R, Driessler F, Macia L, Khor EC (2014) Pancreatic polypeptide controls energy homeostasis via Npy6r signaling in the suprachiasmatic nucleus in mice. Cell Metab 19(1):58–72.  https://doi.org/10.1016/j.cmet.2013.11.019 CrossRefGoogle Scholar
  45. Zendehdel M, Babapour V, Asadi S (2009) Effects of intracerebroventricular injections of glucose and insulin on food intake in broiler cockerels. Anim Sci J 22(1):6–12 (Persian) Google Scholar
  46. Zendehdel M, Mokhtarpouriani K, Babapour V, Pourrahimi M, Hamidi F (2013) The role of 5-HT2A and 5-HT2C receptors on harmaline induced eating behavior in 24-h food-deprived broiler cockerels. IJVR 14(2):94–99Google Scholar
  47. Zendehdel M, Hamidi F, Hassanpour S (2015) The effect of histaminergic system on nociceptin/orphanin FQ induced food intake in chicken. Int J Pept Res Ther 21(2):179–186.  https://doi.org/10.1007/s10989-014-9450-8 CrossRefGoogle Scholar
  48. Zendehdel M, Parvizi Z, Hassanpour S, Baghbanzadeh A, Hamidi F (2017) Interaction between nociceptin/orphanin FQ and adrenergic system on food intake in neonatal chicken. Int J Pept Res Ther 23(1):155–161.  https://doi.org/10.1007/s10989-016-9548-2 CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Basic Sciences, Faculty of Veterinary MedicineFerdowsi University of MashhadMashhadIran
  2. 2.Department of Basic Sciences, Faculty of Veterinary MedicineUniversity of TehranTehranIran

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