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Immobilized Peptide on the Surface of Poly l-DOPA/Silica for Targeted Delivery of 5-Fluorouracil to Breast Tumor

  • Maryam Nazemian
  • Vida HojatiEmail author
  • Saeed Zavareh
  • Hamid Madanchi
  • Hamid Hashemi-MoghaddamEmail author
Article
  • 7 Downloads

Abstract

Chemotherapy using drug delivery systems can target tumor cells selectively and do not affect normal cells. In this paper, a specific drug delivery system with immobilized NL2 peptide on the surface of polymeric nano drug was designed for treatment of breast tumor. The tertiary structure of NL2 peptide (AEGEFIHNRYNRFFYWYGDPAK) was selected from the database and synthesized. After that, it was coupled to the synthesized poly 3,4-dihydroxy-l-phenylalanine (DOPA)/SiO2 nano-composite and examined for targeted 5-fluorouracil (5-FU) delivery in nude mice bearing MCF7 human breast carcinoma cells. Investigation of drug deposition in different tissue and in vivo experiments showed drug in combination with functionalized nano-composite with peptide (DPP) improve targeting properties of drug and 5-FU concentration was significantly increased in tumor rather than other tissues. In addition, this functionalization increases antitumor and targeting efficacy than the free 5-FU and non-functionalized nanocomposite.The poly DOPA functionalized peptide is a powerful agent for targeted delivery of anticancer drugs such as 5FU to tumor cells.

Keywords

5-Fluorouracil Poly 3,4-dihydroxy-l-phenylalanine Anti-cancer peptide Breast cancer 

Notes

Acknowledgements

The authors are grateful for the MCF7 cell line given by the Pasteur Institute of Iran.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

All experiments were performed according to the The International Guiding Principles for Biomedical Research Involving Animals and were approved by the Institutional Bioethics Commission. This investigation does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biology, Damghan BranchIslamic Azad UniversityDamghanIran
  2. 2.School of BiologyDamghan UniversityDamghanIran
  3. 3.Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research CenterPasteur Institute of IranTehranIran
  4. 4.Department and Center for Biotechnology ResearchSemnan University of Medical SciencesSemnanIran
  5. 5.Department of Chemistry, Damghan BranchIslamic Azad UniversityDamghanIran

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