In Silico Analysis for Determination and Validation of Iron-Regulated Protein from Escherichia coli

  • Fateme Sefid
  • Armina Alagheband Bahrami
  • Maryam Darvish
  • Robab Nazarpour
  • Zahra PayandehEmail author


The iron ion is an essential element in biological processes. Many of biological activities in cells, such as peroxide reduction, nucleotide biosynthesis, and electron transport, are helped via iron ions. Extra-intestinal localities have few iron content; so that, during the infection period, the ExPEC strain attempts to pick up iron from the host. The ireA gene is an iron-regulated gene and is involved in iron attainment in human pathogenic E. coli isolates. A better understanding of the essence of ireA as well as its role in serious E. coli infections will help to provide a new and more effective treatment for E. coli infections. Knowledge of the three-dimensional structure of proteins can contribute to the fraction of their function, as well as their interactions with other compounds such as ligands. In addition, rational modification and protein engineering depend on identification of their 3D structures. Thereafter, various bioinformatics tools were employed to predict their immunological, biochemical and functional properties. Our results indicated that this modeled protein form common beta barrel structures. Our immunological, biochemical and functional analysis have led us to select a region of each antigen harboring the highest immunogenic properties. Our strategy to employ 3D structure prediction and epitope prediction results could be deemed as an amenable approach for efficient vaccine design. Our strategy could pave the way for further structural, functional and therapeutic studies in the context of vaccine design investigations.


Urinary tract infections Vaccine Iron receptor Bioinformatics OMP 



The authors thank Yazd University of Medical Sciences and Tabriz University of Medical Sciences for support to conduct this work.

Compliance with Ethical Standards

Conflict of interest

The Authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Fateme Sefid
    • 1
    • 2
  • Armina Alagheband Bahrami
    • 3
  • Maryam Darvish
    • 4
  • Robab Nazarpour
    • 5
  • Zahra Payandeh
    • 6
    Email author
  1. 1.Departeman of Medical GeneticsShahid Sadoughi University of Medical ScienceYazdIran
  2. 2.Departeman of BiologyScience and Art UniversityYazdIran
  3. 3.Department of Biotechnology, School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran
  4. 4.Departeman of Medical Biotechnology, Faculty of MedicineArak University of Medical ScienceArākIran
  5. 5.Biotechnology Research CenterTabriz University of Medical ScienceTabrizIran
  6. 6.Immunology Research CenterTabriz University of Medical SciencesTabrizIran

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