Antioxidative Role of Buffalo (Bubalus bubalis) Colostrum Whey Derived Peptides During Oxidative Damage
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Nutritional and functional properties of dietary proteins are attributed to physiologically active peptides encrypted within them. In the present study, we focused on whey proteins from buffalo colostrum and analyzed the effect of peptides derived from peptic-digestion on DNP-induced oxidative stress on blood components. Peptides with multifunctional properties were predicted through the BIOPEP database. Free and transglutaminase-interlinked peptides were tested for their efficacy as antioxidants. Free peptides were found to be proficient quenchers compared to interlinked peptides. The hydrolysate (IC50-481 µg) and its permeate (< 3 kDa; IC50–505 µg) were effective in bringing elevated levels of ROS, H2O2 & catalase back to normal levels. Additionally, it also replenished the depleted levels of blood GSH and moderately restored lysosomal enzyme activities. The lead peptide-YVHPFHL sequence with the key terminal HL domain (IC50 339 ± 2 µM) obtained from permeate further substantiated the antioxidative potency of colostrum peptides. Hence, we propose that whey-based peptides can be leveraged for the production of functional foods to treat diverse physiological anomalies.
KeywordsBuffalo colostrum Whey proteins Peptides BIOPEP DNP Oxidative stress
Author acknowledges Departmental UGC-SAP-DSA phase II (No. F.4-1/2013) project. Ashok N R thanks UGC, India for award of RGNF fellowship and Institution of Excellence, University of Mysore, Mysore.
Compliance with Ethical Standards
Conflict of interest
Ashok N R, Vivek K H and Aparna H S declare that they have no conflict of interest.
All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee.
Informed consent was obtained from the individual participants included in the study.
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