Studies on the Production of Broad Spectrum Antimicrobial Compound Polypeptide (Actinomycins) and Lipopeptide (Fengycin) from Streptomyces sp. K-R1 Associated with Root of Abutilon indicum against Multidrug Resistant Human Pathogens
Endophytic actinomycetes associated with medicinal plants of Chhattisgarh are rich source of novel antimicrobial compounds. The aim of this study was to evaluate the production of antibiotics by endophytic Streptomyces sp. K-R1 associated with root of Abutilon indicum against multidrug resistant human pathogens. The 16S rRNA sequence of the isolate K-R1 has been identified as Streptomyces parvulus NBRC 13193T (AB184326) with the GenBank database (accession number KY777591). Streptomyces parvulus K-R1 grown under submerged fermentation condition optimized by central composite design (glucose—11.25 g/l, soybean meal 12.17 g/l, calcium carbonate—1.47 g/l, sodium chloride—11.25 g/l; pH 7.18 at 30.51 °C with 4% seed inoculum for 10 days of incubation) exhibited highest activity against multidrug resistant Staphylococcus aureus JNMC-3, Staphylococcus epidermidis JNMC-4, Klebsiella pneumoniae MTCC-3384, Klebsiella pneumoniae JNMC-6, Pseudomonas aeruginosa MTCC-741, Proteus vulgaris JNMC-7, Candida albicans MTCC-183 and Aspergillus niger MTCC-872. The structures of the active compounds were elucidated by UV–Vis spectroscopy, 1H and 13C NMR, FTIR and ESIMS. Actinomycin D, actinomycin X0β and fengycin were detected in crude extracts and major components were eluted by HPLC at 10.63, 10.88 and 6.3 min respectively. In this case, a high yield of antibiotics was achieved with Streptomyces parvulus K-R1 fermented in glucose soybean meal broth media with a production of 240 mg/l after 5 days, 290 mg/l after 8 days and 340 mg/l after 11 days, which can be used in industrial fermentation process to obtain high yields. To the author’s best knowledge, it is the first report of fengycin production by Streptomyces parvulus.
KeywordsEndophytic actinomycetes Streptomyces parvulus Antibiotic production Multidrug resistant pathogens Optimization Central composite design
Authors are thankful to the Head, SLS, PRSU, Raipur for providing the necessary facilities for the research. One of the authors (SC) is grateful to University Grant Commission, New Delhi, India for financial support in the form of Basic Science Research (F.7-145/2007BSR), SRF. Financial support from DST under FIST program and UGC for DRS-SAP III are gratefully acknowledged. The authors also thank Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh for providing cultures and Pandit Jawaharlal Nehru Medical College, Raipur, Chhattisgarh for providing clinical cultures for research.
This study was funded by University Grant Commission, New Delhi, India (F.7-145/2007BSR).
Compliance with Ethical Standards
Conflict of interest
Authors declares that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
- Castillo U, Harper JK, Strobel GA, Sears J, Alesi K, Ford E, Lin J, Hunter M, Maranta M, Ge H, Yaver D, Jensen JB, Porter H, Robison R, Miller D, Hess WM, Condron M, Teplow D (2003) Kakadumycins, novel antibiotics from Streptomyces sp. NRRL 30566, an endophyte of Grevillea pteridifolia. FEMS Microbiol Lett 234:183–190CrossRefGoogle Scholar
- Chandrakar S, Gupta A (2017) Antibiotic potential of endophytic actinomycetes of medicinal herbs against human pathogenic bacteria. Proc Natl Acad Sci India B 87:905–915Google Scholar
- Chen C, Song F, Wang Q, Abdel-Mageed WM, Guo H, Fu C, Hou W, Dai H, Liu X, Yang N, Xie F, Yu K, Chen R, Zhang L (2012) A marine-derived Streptomyces sp. MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity. Appl Microbiol Biotech. https://doi.org/10.1007/s00253-012-4079-z CrossRefGoogle Scholar
- Ezra D, Castillo UF, Strobel GA, Hess WM, Porter H, Jensen JB, Condron MAM, Teplow DB, Sears J, Maranta M, Hunter M, Weber B, Yaver D (2004) Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp. Microbiology 150:785–793CrossRefPubMedGoogle Scholar
- Hamza AA, Ali HA, Clark BR, Murphy CD, Elobaid EA (2013) Isolation and characterization of actinomycin D producing Streptomyces sp. from Sudanese soil. Afr J Biotechnol 12:2624–2632Google Scholar
- Katz E, Pienta P, Sivak A (1956) The role of nutrition in the synthesis of actinomycin. Appl Microbiol 6:236–241Google Scholar
- Kawato M, Shinobu R (1959) Cover slip culture of Streptomyces herbaricolour nov. sp. supplement: a simple technique for the microscopical observation. Mem Osaka Univ Lib Arts Educ 8:114–119Google Scholar
- Khieu TN, Liu MJ, Nimaichand S, Quach NT, Ky SC, Ph QT, Vu TT, Nguyen TD, Xiong Z, Prabhu DM, Li WJ (2015) Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour. Front Microbiol. https://doi.org/10.3389/fmicb.2015.00574 PubMedPubMedCentralCrossRefGoogle Scholar
- Kulkarni M, Gorthi S, Banerjee G, Chattopadhyay P (2017) Production, characterization and optimization of actinomycin D from Streptomyces hydrogenans IB310, a (n antagonistic bacterium against phytopathogens). Biocatal Agric Biotechnol 10:69–74Google Scholar
- Nafis A, Oubaha B, Elhidar N, Ortlieb N, Kulik A, Niedermeyer T, Hassani L, Barakate M (2018) Novel production of two new non polyenic antifungal macrolide derivatives by Streptomyces Z26 isolated from Moroccan rhizospheric soil. Online J Biol Sci. https://doi.org/10.3844/ojbsci.2018 CrossRefGoogle Scholar
- Rahman MA, Islam MZ, Khondkar P, Islam MA (2010) Characterization and antimicrobial activities of a polypeptide antibiotic isolated from a new strain of Streptomyces parvulus. Bangla Pharma J 13:14–17Google Scholar
- Salam N, Khieu TN, Liu MJ, Vu TT, Ky SC, Quach NT, Phi QT, Narsing Rao MP, Fontana A, Sarter S, Li WJ (2017) Endophytic actinobacteria associated with Dracaena cochinchinensis Lour: isolation, diversity and their cytotoxic activities. BioMed Res Int. https://doi.org/10.1155/2017/1308563 PubMedPubMedCentralCrossRefGoogle Scholar
- Solanki R, Kundu A, Das P, Khanna M (2015) Characterization of antimicrobial compounds from Streptomyces sp. World J Pharma Res 4:1626–1641Google Scholar
- Taechowisan T, Chanaphat S, Ruensamran W, Phutdhawong SW (2014) Antibacterial activity of new flavonoids from Streptomyces sp. BT01; an endophyte in Boesenbergia rotunda (L) Mansf. J Appl Pharma Sci 4:8–13Google Scholar
- Usha R, Ananthaselvi P, Venil CK, Palaniswamy M (2010) Antimicrobial and antiangiogenesis activity of Streptomyces parvulus KUAP106 from mangrove soil. Eur J Biol Sci 2:77–83Google Scholar