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Studies on the Production of Broad Spectrum Antimicrobial Compound Polypeptide (Actinomycins) and Lipopeptide (Fengycin) from Streptomyces sp. K-R1 Associated with Root of Abutilon indicum against Multidrug Resistant Human Pathogens

  • Sandhya Chandrakar
  • Ashwini Kumar Gupta
Article

Abstract

Endophytic actinomycetes associated with medicinal plants of Chhattisgarh are rich source of novel antimicrobial compounds. The aim of this study was to evaluate the production of antibiotics by endophytic Streptomyces sp. K-R1 associated with root of Abutilon indicum against multidrug resistant human pathogens. The 16S rRNA sequence of the isolate K-R1 has been identified as Streptomyces parvulus NBRC 13193T (AB184326) with the GenBank database (accession number KY777591). Streptomyces parvulus K-R1 grown under submerged fermentation condition optimized by central composite design (glucose—11.25 g/l, soybean meal 12.17 g/l, calcium carbonate—1.47 g/l, sodium chloride—11.25 g/l; pH 7.18 at 30.51 °C with 4% seed inoculum for 10 days of incubation) exhibited highest activity against multidrug resistant Staphylococcus aureus JNMC-3, Staphylococcus epidermidis JNMC-4, Klebsiella pneumoniae MTCC-3384, Klebsiella pneumoniae JNMC-6, Pseudomonas aeruginosa MTCC-741, Proteus vulgaris JNMC-7, Candida albicans MTCC-183 and Aspergillus niger MTCC-872. The structures of the active compounds were elucidated by UV–Vis spectroscopy, 1H and 13C NMR, FTIR and ESIMS. Actinomycin D, actinomycin X and fengycin were detected in crude extracts and major components were eluted by HPLC at 10.63, 10.88 and 6.3 min respectively. In this case, a high yield of antibiotics was achieved with Streptomyces parvulus K-R1 fermented in glucose soybean meal broth media with a production of 240 mg/l after 5 days, 290 mg/l after 8 days and 340 mg/l after 11 days, which can be used in industrial fermentation process to obtain high yields. To the author’s best knowledge, it is the first report of fengycin production by Streptomyces parvulus.

Keywords

Endophytic actinomycetes Streptomyces parvulus Antibiotic production Multidrug resistant pathogens Optimization Central composite design 

Notes

Acknowledgements

Authors are thankful to the Head, SLS, PRSU, Raipur for providing the necessary facilities for the research. One of the authors (SC) is grateful to University Grant Commission, New Delhi, India for financial support in the form of Basic Science Research (F.7-145/2007BSR), SRF. Financial support from DST under FIST program and UGC for DRS-SAP III are gratefully acknowledged. The authors also thank Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh for providing cultures and Pandit Jawaharlal Nehru Medical College, Raipur, Chhattisgarh for providing clinical cultures for research.

Funding

This study was funded by University Grant Commission, New Delhi, India (F.7-145/2007BSR).

Compliance with Ethical Standards

Conflict of interest

Authors declares that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

10989_2018_9727_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1097 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Microbiology Research Laboratory, School of Studies in Life SciencePt. Ravishankar Shukla UniversityRaipurIndia

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