Identification of an Ultra-Short Peptide with Potent Pseudomonas aeruginosa Activity for Development as a Topical Antibacterial Agent

  • Shu Wei Teo
  • Yaqing Elena Yong
  • Siew Mei Samantha Ng
  • Fui Mee Ng
  • Jeanette Woon Pei Teo
  • Roland Jureen
  • Jeffrey Hill
  • C. S. Brian ChiaEmail author
Short Communication


Pseudomonas aeruginosa is a Gram-negative pathogen responsible for a wide spectrum of infections including skin-related infections such as ecthyma gangrenosum, folliculitis, surgical site and burn wound infections. The global emergence of drug-resistant strains has become a clinical concern and new antibacterial agents are urgently needed. Antimicrobial peptides, with their membrane-disrupting mode of action, are deemed potential candidates. Herein, we screened 62 published short peptides, up to 11 residues in length, with reported antibacterial properties, against a multidrug-resistant strain of P. aeruginosa. Of the 3 most potent peptides, the shortest one was selected for further studies involving a panel of clinical P. aeruginosa strains, a bactericidal/static determination assay and a time-kill assay to gauge its potential for further development as a topical antibacterial drug candidate.


Antimicrobial peptides Broad-spectrum antibiotic Pseudomonas aeruginosa 



The authors thank the Agency for Science, Technology and Research Biomedical Research Council for funding.

Compliance with Ethical Standards

Conflict of interest

The authors of this paper declare no conflicts of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR)SingaporeSingapore
  2. 2.Department of Laboratory MedicineNational University HospitalSingaporeSingapore

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