Inhibition of Methicillin Resistant Staphylococcus aureus by Bacteriocin Producing Pseudomonas aeruginosa
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Bacteriocins are natural antimicrobial peptides with attractive possible applications in food preservation and health care. In the present study, bacteriocin producing bacterial strain Pseudomonas aeruginosa were isolated from soil which exhibited antagonistic activity against Methicillin Resistant Staphylococcus aureus (MRSA) bacteria. The bacteriocin producing strain TA6 was confirmed as P. aeruginosa by biochemical tests and 16S rRNA gene sequence analysis. Maximum bacteriocin activity (100 AU ml−1) was observed at 37 °C with pH 6.0 in 24 h time duration. SDS–PAGE analysis of the extracellular protein of P. aeruginosa TA6 revealed a bacteriocin-like protein with a molecular mass of ~10 kDa. MRSA cells were treated with culture supernatant of P. aeruginosa TA6 and analyzed by FT-IR. The treated and untreated MRSA showed band variations at 671 and 3460 cm−1 corresponding to alkyl and amide group respectively. Mixed proportions of dead and live control populations were analyzed by flow cytometry to determine detection limits of the Dead/Live cells. The flow cytometry detection of defined proportions of dead (p2) and live (p1) cells at 3 h were p2 = 60.5%; p1 = 39.5% and 6 h p2 = 66.5%; p1 = 33.5% respectively. The scanning electron microscopy observation showed the main changes in the cell membrane structural integrity of S. aureus after exposure to the bacteriocin from P. aeruginosa TA6 at 12 h incubation. Together, the results suggested that bacteriocin from P. aeruginosa TA6 was effective against MRSA.
KeywordsBacteriocin Methicillin-resistant Staphylococcus aureus Pseudomonas aeruginosa TA6 SDS–PAGE SEM
The authors gratefully acknowledge the Department of Science and Technology, New Delhi for providing financial supports under DST-WOS-A start of grant for (DST/SR/WOS- A/LS-629/2012(G)), Scheme.
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Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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