Inhibition of Methicillin Resistant Staphylococcus aureus by Bacteriocin Producing Pseudomonas aeruginosa

  • Thangarasu Arumugam
  • Selvam Dhanam
  • Neelamegam Rameshkumar
  • Muthukalingan Krishnan
  • Nagarajan KayalvizhiEmail author


Bacteriocins are natural antimicrobial peptides with attractive possible applications in food preservation and health care. In the present study, bacteriocin producing bacterial strain Pseudomonas aeruginosa were isolated from soil which exhibited antagonistic activity against Methicillin Resistant Staphylococcus aureus (MRSA) bacteria. The bacteriocin producing strain TA6 was confirmed as P. aeruginosa by biochemical tests and 16S rRNA gene sequence analysis. Maximum bacteriocin activity (100 AU ml−1) was observed at 37 °C with pH 6.0 in 24 h time duration. SDS–PAGE analysis of the extracellular protein of P. aeruginosa TA6 revealed a bacteriocin-like protein with a molecular mass of ~10 kDa. MRSA cells were treated with culture supernatant of P. aeruginosa TA6 and analyzed by FT-IR. The treated and untreated MRSA showed band variations at 671 and 3460 cm−1 corresponding to alkyl and amide group respectively. Mixed proportions of dead and live control populations were analyzed by flow cytometry to determine detection limits of the Dead/Live cells. The flow cytometry detection of defined proportions of dead (p2) and live (p1) cells at 3 h were p2 = 60.5%; p1 = 39.5% and 6 h p2 = 66.5%; p1 = 33.5% respectively. The scanning electron microscopy observation showed the main changes in the cell membrane structural integrity of S. aureus after exposure to the bacteriocin from P. aeruginosa TA6 at 12 h incubation. Together, the results suggested that bacteriocin from P. aeruginosa TA6 was effective against MRSA.


Bacteriocin Methicillin-resistant Staphylococcus aureus Pseudomonas aeruginosa TA6 SDS–PAGE SEM 



The authors gratefully acknowledge the Department of Science and Technology, New Delhi for providing financial supports under DST-WOS-A start of grant for (DST/SR/WOS- A/LS-629/2012(G)), Scheme.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Thangarasu Arumugam
    • 1
  • Selvam Dhanam
    • 1
  • Neelamegam Rameshkumar
    • 2
  • Muthukalingan Krishnan
    • 2
  • Nagarajan Kayalvizhi
    • 1
    Email author
  1. 1.Department of Zoology, School of Life SciencesPeriyar UniversitySalemIndia
  2. 2.Insect Molecular Biology Laboratory, Department of Environmental BiotechnologyBharathidasan UniversityTiruchirappalliIndia

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